Anti-ERG Rabbit monoclonal Antibody for WB, IHC-P, ELISA - P11308

Core Product Specifications and Parameters

Biological Background: Transcriptional regulator ERG Function and Localization

• ERG (ETS-related gene) is a transcriptional regulator that may modulate gene expression through recruitment of SETDB1 histone methyltransferase, influencing local chromatin structure.
• It belongs to the ETS family of transcription factors and is also known as transforming protein ERG.
• ERG localizes to both the nucleus and cytoplasm, consistent with its role in transcriptional regulation and potential signaling functions.
• The protein undergoes alternative splicing, producing multiple isoforms, and is subject to post-translational modifications including phosphorylation and ubiquitination (isopeptide bond).
• ERG is frequently involved in chromosomal rearrangements in various cancers, notably prostate cancer, where TMPRSS2-ERG gene fusions drive oncogenesis.
• Keywords: 3D-structure, Alternative splicing, Chromosomal rearrangement, Cytoplasm, DNA-binding, Isopeptide bond, Nucleus, Phosphoprotein, Proteomics identification, Proto-oncogene, Reference proteome, Transcription, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 379-479 of human ERG (NP_891548.1). This region may be present in multiple isoforms; verify detection specificity in your experimental system.
• For Western blot, a band around 54 kDa is expected; optimize lysate preparation and antibody dilution for your sample type.
• In IHC-P, antigen retrieval and detection conditions should be optimized; consider including known positive and negative tissue controls.
• ELISA applications may require pairing with a suitable capture/detection antibody; validate assay performance using recombinant ERG protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials. This Anti-ERG Rabbit mAb supports cancer research and assay development, and our comprehensive portfolio includes validated antibody pairs, bulk ancillary reagents, and sourcing for rare targets. Partner with us to accelerate your translational workflow from concept to clinic.