Anti-eRF3A/GSPT1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15170

Core Product Specifications and Parameters

Biological Background: eRF3A/GSPT1 Function and Localization

• eRF3A/GSPT1, also known as GST1, ETF3A, eRF3a, or 551G9.2, is a eukaryotic peptide chain release factor GTP-binding subunit (ERF3A) encoded by the GSPT1 gene in humans.
• Functions as a GTPase component of the eRF1-eRF3-GTP ternary complex mediating translation termination at stop codons UAA, UAG, and UGA. Related references: PMID:15987998, PMID:19417105, PMID:2511002
• Mediates delivery of ETF1/ERF1 to stop codons; GTP hydrolysis induces conformational change leading to dissociation of GSPT1/ERF3A, allowing ETF1 to accommodate fully in the ribosomal A-site. Related references: PMID:27863242, PMID:16777602
• Acts as a component of the transient SURF complex that recruits UPF1 to stalled ribosomes, facilitating nonsense-mediated mRNA decay (NMD) of transcripts containing premature stop codons. Related references: PMID:24486019
• Required for SHFL-mediated translation termination that inhibits programmed ribosomal frameshifting (-1PRF) in both viral and cellular genes. Related references: PMID:30682371
• Keywords associated with GSPT1 include GTP-binding, hydrolase activity, nucleotide-binding, protein biosynthesis, alternative splicing, and nonsense-mediated mRNA decay.
• Predicted molecular weight is approximately 56 kDa (calculated 55.7 kDa), consistent with the UniProt annotation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1–100) of human eRF3A/GSPT1 (NP_001123479.1), which may confer specificity to the N-terminus of the protein.
• Given the broad reactivity (Human, Rat), validate the antibody in relevant sample systems using appropriate controls, especially if using for species not explicitly listed.
• Expected band size in Western blot is ~56 kDa; minor bands of different sizes may correspond to isoforms generated by alternative splicing.
• For immunofluorescence or immunohistochemistry, consider optimizing fixation and permeabilization conditions, as the target is predominantly cytoplasmic.
• This product is validated for WB, IF/ICC, IF-P, IHC-P, and ELISA; ensure individual protocol optimization for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with reliable access to antibodies and reagents for every stage of development. The eRF3A/GSPT1 monoclonal antibody supports research in translational control, nonsense-mediated mRNA decay, and related disease areas. Beyond single products, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets to streamline your assay development workflow.