Anti-ERCC6L2 Rabbit Polyclonal Antibody for WB and ELISA - Q5T890

Core Product Specifications and Parameters

Biological Background: ERCC6L2 Function and Localization

• ERCC6L2 promotes efficient DNA double-strand break (DSB) end-joining and facilitates programmed recombination by controlling the spatial orientation of DNA ends. It also restricts DNA end resection during DSB repair.
  Related references: PMID:24507776, PMID:37014751
• Required for the smooth replication of complex genomic regions and contributes to the maintenance of chromatin structure.
  Related references: PMID:37014751
• Encoded by the ERCC6L2 gene (alternative names C9orf102, RAD26L), the protein is also referred to as DNA repair and recombination protein RAD26-like and carries alias designations HEBO, BMFS2, and SR278.
• Localizes to the nucleus, cytoplasm, centrosome, mitochondrion, and chromosome centromeres, reflecting its involvement in DNA repair and cell cycle regulation.
• Contains ATP-binding and helicase domains, DNA-binding motifs, and is classified as a hydrolase. It is a phosphoprotein, indicating post-translational regulation.
• Expressed at the protein level in bone marrow, suggesting a role in hematopoietic development or maintenance.

Experimental Guidance and Technical Tips

• This polyclonal antibody is generated against a C-terminal recombinant fragment spanning amino acids 1262–1561 of human ERCC6L2; consider the immunogen region when planning detection experiments.
• Validated for Western blot (WB) and ELISA; for WB, given the predicted molecular weight of ~177 kDa, use an appropriate low-percentage polyacrylamide gel and extended transfer times to ensure efficient transfer.
• The antibody cross-reacts with human and mouse ERCC6L2; validate species-specific reactivity in your sample system prior to quantitative assays.
• As a rabbit polyclonal preparation, lot-to-lot variability may occur; titrate the antibody for each new batch and include relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. For DNA repair–focused projects, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies like this ERCC6L2 rabbit pAb, bulk ancillary reagents, and support for rare-target sourcing. Leverage our integrated supply network to accelerate your assay development from concept to clinic.