Anti-ERCC3 Rabbit Polyclonal Antibody for WB, ELISA - P19447

Core Product Specifications and Parameters

Biological Background: ERCC3 Function and Localization

• ERCC3 (XPB) is the 89 kDa subunit of the general transcription and DNA repair factor IIH (TFIIH) core complex, also known as basic transcription factor 2 89 kDa subunit (BTF2 p89).
• It functions as an ATP-dependent 3'-5' DNA helicase/translocase that binds dsDNA and unzips it via translocase activity rather than classical helicase action. Related references:
  PMID:27193682, PMID:33902107
• In transcription initiation, ERCC3 is essential for promoter opening and escape; its ATP-dependent helicase activity induces and propagates a DNA twist to open the promoter. Related references:
  PMID:10024882, PMID:8157004, PMID:27193682, PMID:33902107
• In nucleotide excision repair (NER), ERCC3 helps open DNA around lesions; the ATPase activity (but not helicase activity) is required for initial DNA melting, while XPA retains it at the 5' end of a DNA bubble. Related references:
  PMID:17466626, PMID:2111438, PMID:31253769
• ERCC3 is located exclusively in the nucleus.
• It participates in host-virus interactions and is linked to diseases such as xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy when mutated.
• Post-translational modifications include phosphorylation; it is a phosphoprotein.
• The protein has a molecular weight of 89,278 Da and is involved in critical cellular processes including transcription regulation, DNA repair, and DNA damage response.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 512–782 of human ERCC3 (NP_000113.1); this region may generate antibodies recognizing both full-length ERCC3 and potential isoforms.
• For Western blotting, consider using nuclear extracts or whole-cell lysates from human, mouse, or rat cell lines; the predicted band size is approximately 89 kDa.
• ELISA can be employed for quantifying ERCC3 levels or screening, but optimize coating and detection conditions for your specific assay format.
• Since ERCC3 is a component of the TFIIH complex, co-immunoprecipitation experiments may require careful optimization to maintain complex integrity.
• When studying DNA repair or transcription, validate the antibody's performance in your relevant sample system under induced or baseline conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. For researchers investigating transcription and DNA repair pathways, we provide reliable anti-ERCC3 antibodies and can assist with the sourcing of related components to accelerate your assay development from concept to clinic.