Anti-ErbB4/HER4 Rabbit Polyclonal Antibody for WB and ELISA - Q15303

Core Product Specifications and Parameters

Biological Background: ErbB4/HER4 Function and Localization

• Receptor tyrosine-protein kinase erbB-4, also known as HER4 or p180erbB4, belongs to the epidermal growth factor receptor (EGFR/ERBB) family.
• Functions as a cell surface receptor for neuregulins (NRG1-4) and EGF family members (BTC, EREG, HBEGF), triggering dimerization, autophosphorylation, and downstream signaling cascades.
• Plays essential roles in cardiac muscle differentiation, embryonic central nervous system development (neural crest migration, axon guidance), and mammary gland differentiation and lactation.
• Alternative splicing generates isoforms JM-A and JM-B, each with CYT-1 and CYT-2 variants; isoform JM-A undergoes proteolytic processing, releasing soluble intracellular domains (4ICD) that translocate to the nucleus or mitochondria.
• Nuclear 4ICD colocalizes with STAT5A to activate transcription of milk protein genes; mitochondrial 4ICD promotes apoptosis.
• Isoform-specific signaling: CYT-1 isoforms activate the PI3K/AKT pathway via PIK3R1 phosphorylation, protecting against apoptosis and promoting migration; CYT-2 isoforms lack the phosphotyrosine for PIK3R1 binding.
• Subcellular localization includes the cell membrane (full-length receptor), nucleus, and mitochondrion (soluble intracellular domains).
• Expressed at highest levels in brain, heart, and kidney; also found in skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis, and breast.
• Post-translational modifications: glycoprotein, phosphoprotein, disulfide bonds, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 1150–1250) lies within the intracellular domain, upstream of the CYT-1/CYT-2 tail variants, and may detect multiple isoforms; consider this when interpreting isoform-specific results.
• For Western blot, the predicted molecular weight is 147 kDa; use appropriate gel percentage (e.g., 6–8% SDS-PAGE) and extended transfer times for efficient blotting of high-molecular-weight proteins.
• Validate the antibody in biologically relevant sample types known to express ErbB4, such as human brain, heart, or breast cancer cell lines, and in cross-reactive rat tissues.
• As a polyclonal antibody, it may exhibit lot-to-lot variability; confirm reactivity and specificity with appropriate positive and negative controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including polyclonal antibodies like this anti-ErbB4/HER4 reagent for signal transduction and oncology studies. Our portfolio encompasses optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for hard-to-find targets, helping you accelerate assay development. From concept to clinic, CamelBio bridges your sourcing needs with reliable, research-grade immunoreagents.