Anti-ErbB4/HER4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15303

Core Product Specifications and Parameters

Biological Background: ErbB4/HER4 Function and Localization

• ErbB4/HER4 is a receptor tyrosine-protein kinase that serves as a cell surface receptor for neuregulins (NRG1-4) and EGF family members (BTC, EREG, HBEGF), playing a pivotal role in signal transduction.
• It regulates critical developmental processes including cardiac muscle differentiation and postnatal cardiomyocyte proliferation, central nervous system development (neural crest cell migration and axon guidance), and mammary gland differentiation and lactation.
• Ligand binding induces receptor dimerization and autophosphorylation, creating docking sites for adaptor proteins and triggering downstream pathways like MAPK/ERK and PI3K/AKT, with isoform-specific signaling outcomes.
• Alternative splicing generates multiple isoforms (e.g., JM-A CYT-1, JM-A CYT-2, JM-B CYT-1, JM-B CYT-2) that differ in their ability to activate PI3K/AKT, protect against apoptosis, and promote cell migration.
• Proteolytic processing of JM-A isoforms releases soluble intracellular domains (4ICD) that translocate to the nucleus to co-activate STAT5A and regulate gene expression (e.g., milk protein CSN2) or to mitochondria to promote apoptosis.
• Subcellularly, ErbB4 localizes primarily to the cell membrane, but after cleavage it can be found in the nucleus and mitochondria.
• Tissue expression is highest in brain, heart, and kidney, with additional expression in skeletal muscle, cerebellum, spleen, testis, and breast; isoform JM-B is predominantly expressed in the heart.
• As a developmental protein and proto-oncogene, ErbB4 is implicated in processes like amyotrophic lateral sclerosis, neurodegeneration, and cancer, making it a key target in signaling and disease research.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1150–1250 of human ErbB4/HER4, a region conserved across human, mouse, and rat, supporting multi-species reactivity.
• For Western blot, the predicted molecular weight is ~147 kDa; consider using appropriate gel percentage and transfer conditions for high molecular weight proteins.
• In IHC-P and IF/ICC, validation in relevant tissue types (brain, heart, breast) is recommended, with isoform-specific detection possible depending on the epitope region.
• Since ErbB4 undergoes proteolytic cleavage, be aware of potential fragments (e.g., 80 kDa 4ICD) in certain assays; include appropriate controls.
• ELISA performance may depend on coating conditions and antigen accessibility; optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development with a comprehensive portfolio of ErbB4/HER4 antibodies and reagents. As your one-stop sourcing partner, we offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents to streamline your assay development from concept to clinic. For rare or custom targets in the ERBB family signaling pathway, our technical team can assist with raw material sourcing.