Anti-ErbB3/HER3 Polyclonal Antibody for WB, IF/ICC, ELISA - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3/HER3 Function and Localization

• ErbB3/HER3 is a receptor tyrosine-protein kinase that serves as a cell surface receptor for neuregulins, especially NRG1, and ligand binding induces tyrosine phosphorylation and PI3K pathway activation. Related references: PMID:20682778
• It can also be activated by the neuroglycan CSPG5, expanding its regulatory roles. Related references: PMID:15358134
• The protein is involved in the regulation of myeloid cell differentiation, linking it to hematopoietic processes. Related references: PMID:27416908
• ErbB3/HER3 is predominantly expressed in epithelial tissues and the brain, consistent with its roles in development and cancer.
• It localizes at the cell membrane and is also secreted, allowing both membrane-bound and soluble signaling modalities.
• The protein undergoes alternative splicing, glycosylation, and phosphorylation, which modulate its function and interactions.
• As a proto-oncogene, ErbB3/HER3 is frequently implicated in oncogenic signaling, making it a key target in cancer research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20-285) of human ErbB3/HER3, targeting the ligand-binding region. This design supports detection of both full-length and potentially shed forms.
• For Western blot, a band around 148 kDa is expected; consider using appropriate protein ladders and validate in your target cell lines (e.g., MCF-7, A549) known to express ErbB3/HER3.
• In immunofluorescence/ICC, fixation and permeabilization conditions should be optimized; membrane localization is anticipated.
• For ELISA, the recombinant immunogen may serve as a positive control; ensure antibody titration for optimal signal-to-noise ratio.
• Due to cross-reactivity with mouse and rat, this antibody can be used for comparative oncology studies across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibodies for key oncology targets such as ErbB3/HER3. Our portfolio covers optimized polyclonal and monoclonal antibodies, antigen pairs, and bulk ancillary reagents, supported by rare-target sourcing capabilities to accelerate your assay development from concept to clinic.