Anti-ErbB3/HER3 Monoclonal Antibody for Flow Cytometry - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3/HER3 Function and Localization

• Receptor tyrosine-protein kinase erbB-3 (ErbB3/HER3) functions as a cell surface receptor for neuregulins, primarily neuregulin-1 (NRG1). Related references: PMID:20682778
• Ligand binding induces tyrosine phosphorylation of ErbB3, promoting its association with the p85 regulatory subunit of phosphoinositide 3-kinase (PI3K) and activation of downstream signaling. Related references: PMID:20682778
• ErbB3 may also be activated by chondroitin sulfate proteoglycan 5 (CSPG5), indicating alternative regulatory mechanisms. Related references: PMID:15358134
• The protein plays a role in the regulation of myeloid cell differentiation. Related references: PMID:27416908
• Encoded by the ERBB3 gene, multiple alternatively spliced transcript variants exist, giving rise to isoforms including secreted forms (e.g., p45-sErbB3, p85-sErbB3).
• Subcellular localization includes the cell membrane, but secreted isoforms lacking the transmembrane domain are also generated.
• Expression is primarily observed in epithelial tissues and brain, with implications in development and oncogenesis.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation, as annotated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen encompasses the entire extracellular domain (aa 20-643), so the antibody likely recognizes an epitope in the extracellular region, making it suitable for detecting cell surface ErbB3 by flow cytometry.
• For FC, optimize the antibody dilution using positive control cells such as human epithelial lines (e.g., MCF-7, A431) known to express ErbB3.
• Since ErbB3 may be shed, consider including a wash step and avoid excessive trypsinization to preserve surface epitopes.
• Validate staining specificity by knockdown or knockout controls in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For ErbB3/HER3 research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies including conjugated formats, bulk ancillary reagents, and support for rare target raw-material sourcing. Our portfolio streamlines procurement for cancer biomarker studies, companion diagnostics, and receptor signaling workflows.