Anti-ERBB2IP Rabbit Polyclonal Antibody for WB, ELISA - Q96RT1

Core Product Specifications and Parameters

Biological Background: Erbin Function and Localization

• Erbin (also known as Densin-180-like protein, Erbb2-interacting protein, Protein LAP2) is encoded by the ERBIN (ERBB2IP) gene and exists in alternatively spliced isoforms.
• Erbin functions as an adaptor protein for the receptor tyrosine kinase ERBB2 in epithelial cells, binding to the unphosphorylated Tyr-1248 residue and potentially stabilizing the receptor's inactive state. Related references: PMID:16203728
• Erbin inhibits NOD2-dependent NF-κB signaling and suppresses pro-inflammatory cytokine secretion. Related references: PMID:16203728
• In keratinocytes, Erbin regulates differentiation by binding and sequestering SHOC2, preventing its participation in RAS-RAF-ERK signaling complexes. Related references: PMID:23524970
• Localizes to multiple cellular compartments: cell junctions (hemidesmosomes), basolateral cell membrane, cytoplasm, and nucleus membrane.
• Highly expressed in brain, heart, kidney, muscle, and stomach; lower levels in liver, spleen, and intestine.
• Contains leucine-rich repeats; is a phosphoprotein with multiple modification sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1152–1371 within the C-terminal region of human Erbin, which may be involved in protein–protein interactions; consider this when interpreting detection patterns.
• For Western blotting, the predicted molecular weight is ~158 kDa; optimize gel percentage (e.g., 6–8% SDS-PAGE) and transfer conditions accordingly.
• Validate antibody specificity using positive (e.g., human epithelial cell lysates) and negative controls; inclusion of a blocking peptide or recombinant protein can confirm target-specific bands.
• In ELISA, the recombinant immunogen can serve as a positive control to establish assay sensitivity and linearity.
• The antibody is polyclonal and may recognize multiple isoforms or degradation fragments; confirm expected band patterns in your experimental system.
• Although validated for WB and ELISA, cross-reactivity with other family members or off-target proteins should be assessed if applied to other techniques.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw material provider, CamelBio offers a comprehensive portfolio of research-grade and diagnostic-grade antibodies, including validated pairs, monoclonal and polyclonal options, and ancillary reagents. For ERBB2IP-related studies—spanning ErbB2 signaling, NF-κB regulation, and keratinocyte biology—CamelBio provides one-stop sourcing from concept to clinic. Our integrated supply chain ensures reliable access to rare-target raw materials and supports bulk custom production, accelerating your assay development and manufacturing workflows.