Anti-ERAP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9NZ08

Core Product Specifications and Parameters

Biological Background: ERAP1 Function and Localization

• ERAP1 (Endoplasmic reticulum aminopeptidase 1) is a critical enzyme in adaptive immunity, trimming antigenic precursor peptides to optimal lengths (8-10 residues) for loading onto MHC class I molecules.
• It exhibits strong substrate length preference, efficiently processing peptides of 9-16 amino acids, and typically stops trimming after generating a 9-mer.
• Preferentially hydrolyzes leucine and peptides with hydrophobic C-termini, while showing weak activity toward charged terminal residues, shaping the immunopeptidome.
• Beyond antigen processing, ERAP1 inactivates angiotensin II and generates bradykinin, suggesting a role in blood pressure regulation within the kidney.
• The protein localizes to the endoplasmic reticulum membrane, anchored by an N-terminal signal peptide and transmembrane domain.
• Ubiquitously expressed across human tissues, reflecting its housekeeping role in peptide turnover.
• Contains key structural features: zinc-binding metalloprotease domain, disulfide bonds, N-glycosylation sites; classified under proteases, hydrolases, and immunity-related proteins.

Experimental Guidance and Technical Tips

• The immunogen (a.a. 842-941) targets a C-terminal region; verify that this epitope is accessible in your target sample under native and denaturing conditions.
• For Western blot, use reducing conditions and expect a band at ~107 kDa; confirm specificity with knockdown or knockout lysates.
• In IHC-paraffin, test antigen retrieval methods (heat-induced, pH 6 or pH 9) as ERAP1 is a membrane protein; counterstain to assess tissue morphology.
• Cross-reacts with mouse and rat ERAP1, but sequence homology in the immunogen region should be checked if using other species; validate in your system.
• ELISA performance depends on coating format; consider using the antibody as capture or detection, and pair with a compatible secondary antibody for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with premium IVD raw materials, technical services, and consultation from concept to clinic. This ERAP1 rabbit monoclonal antibody supports immunological and immuno-oncology research, and is part of our comprehensive portfolio of validated antibodies, bulk ancillary reagents, and custom rare-target sourcing. Rely on our one-stop platform to accelerate your diagnostic development with reliable, production-ready raw materials.