Anti-ERAB/HSD17B10 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99714

Core Product Specifications and Parameters

Biological Background: ERAB/HSD17B10 Function and Localization

• ERAB/HSD17B10 (gene HSD17B10) encodes 3-hydroxyacyl-CoA dehydrogenase type-2, a multifunctional mitochondrial protein also known as ABAD, SCHAD, MRPP2, and 17β-HSD10, with roles in fatty acid β-oxidation, branched-chain amino acid catabolism, and steroid metabolism. Related references: PMID:10600649 PMID:12917011 PMID:18996107 PMID:25925575
• It catalyzes the NAD⁺-dependent oxidation of (S)-3-hydroxyacyl-CoA to 3-ketoacyl-CoA in the mitochondrial fatty acid β-oxidation cycle, with highest activity toward short- and medium-chain substrates. Related references: PMID:10600649 PMID:12917011 PMID:25925575
• It participates in isoleucine degradation by oxidizing 3-hydroxy-2-methylbutyryl-CoA to 2-methyl-3-oxobutyryl-CoA. Related references: PMID:18996107 PMID:19706438 PMID:20077426
• The enzyme exhibits hydroxysteroid dehydrogenase activity, oxidizing various steroid hormones and bile acids at positions 3α, 17β, 20β, 21, and 7α/7β. Related references: PMID:10600649 PMID:12917011 PMID:19706438 PMID:28888424
• It has a phospholipase C-like activity toward cardiolipin and oxidized cardiolipin species, potentially protecting cells from apoptosis during oxidative stress. Related references: PMID:26338420
• Moonlighting as the MRPP2 subunit of mitochondrial ribonuclease P, it is essential for tRNA 5′-end cleavage and, together with MRPP1, catalyzes m¹G9 and m¹A9 tRNA modifications and facilitates 3′-processing and CCA addition. Related references: PMID:18984158 PMID:24549042 PMID:23042678 PMID:29040705 PMID:25925575 PMID:28888424
• The protein localizes to the mitochondrial matrix and nucleoid, and is ubiquitously expressed but overexpressed in neurons of Alzheimer's disease patients. Related references: PMID:9338779 PMID:20077426
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing; disease variants are associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human ERAB/HSD17B10 protein (aa 1-261); for WB, consider testing with mitochondrial-enriched lysates and validate band size against the predicted 27 kDa.
• Given the mitochondrial localization and nucleoid association, fixation and permeabilization protocols for IF/ICC may require optimization to ensure antibody accessibility.
• Cross-reactivity with mouse and rat is reported; confirm the specificity in your model system by including appropriate positive and negative controls, especially when analyzing tissues with high metabolic activity.
• For ELISA, consider pairing with a detection antibody that recognizes a distinct epitope to enhance assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with reliable access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-ERAB/HSD17B10 rabbit pAb, and bulk ancillary reagents. For projects targeting mitochondrial metabolism or neurodegeneration, we offer one-stop sourcing support from concept to clinic, helping you secure rare target reagents and technical resources efficiently. Our supply capabilities encompass custom antibody generation, large-scale production, and rigorous quality control to streamline your assay development.