Anti-Epsin 1 Rabbit Monoclonal Antibody for WB, ELISA - Q9Y6I3

Core Product Specifications and Parameters

Biological Background: Epsin-1 Function and Localization

• Epsin-1, encoded by the EPN1 gene, is an EPS-15-interacting protein also known as EH domain-binding mitotic phosphoprotein. It is a clathrin-associated adaptor protein that plays a key role in clathrin-mediated endocytosis.
• The protein binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), modifies membrane curvature, and facilitates the formation of clathrin-coated invaginations (By similarity).
• Epsin-1 regulates receptor-mediated endocytosis (PubMed:10393179, PubMed:10557078). Related references: PMID:10393179 PMID:10557078
• Epsin-1 localizes to the cytoplasm, cell membrane, nucleus, and specifically clathrin-coated pits, consistent with its role in endocytosis.
• Post-translational modifications include phosphorylation, ubiquitination (Ubl conjugation), and methylation, which may regulate its function and binding interactions.
• Alternative splicing of the EPN1 gene produces multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 400–500 of human Epsin 1 (NP_001123544.1); the antibody may recognize epitopes within this central region.
• For western blotting, consider using whole-cell lysates from human or mouse cell lines. The expected molecular weight is approximately 60 kDa; validate specificity with positive and negative controls and consider pre-adsorption if non-specific bands appear.
• In ELISA applications, titrate the antibody and optimize coating conditions for direct or sandwich formats. Cross-reactivity with mouse Epsin-1 allows testing in mouse samples, but always confirm performance in the relevant tissue or cell system.
• As a monoclonal antibody, lot-to-lot consistency is generally high, but assay conditions should be re-optimized when switching batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and researchers with a one-stop portfolio of IVD raw materials—from validated monoclonal and polyclonal antibodies to bulk ancillary reagents. Our offering includes this anti-Epsin 1 rabbit monoclonal antibody for endocytosis and membrane trafficking studies, and we can source or develop rare-target antibodies and matched antibody pairs tailored to your assay. Backed by technical consultation, CamelBio streamlines procurement from concept to clinic, ensuring reliable access to the raw materials your projects demand.