Anti-EPS8 Polyclonal Antibody for WB, ELISA - Q12929

Core Product Specifications and Parameters

Biological Background: EPS8 Function and Localization

• EPS8 (Epidermal growth factor receptor kinase substrate 8) is a signaling adapter protein that regulates actin cytoskeleton dynamics, controlling processes like cell migration and dendritic cell function. It was initially identified as a substrate for the EGF receptor kinase.
• The protein functions as part of a trimeric complex with SOS1 and ABI1 to transduce signals from Ras to Rac by activating Rac-specific GEF activity, linking growth factor signaling to actin remodeling.
• EPS8 exhibits dual actin-modulating activities: barbed-end capping when bound to ABI1 (inhibiting motility) and actin bundling when associated with BAIAP2 to promote filopodial extensions and membrane protrusions.
• In neurons, EPS8 negatively regulates axonal filopodia formation through actin-capping; however, BDNF-induced phosphorylation inhibits this capping activity, enabling filopodial growth.
• At stereocilia tips in hair cells, EPS8 forms a complex with WHRN and MYO15A that is essential for elongation of the actin core, and mutations in EPS8 are associated with non-syndromic deafness (DFNB102).
• Subcellularly, localizes to the cell cortex, ruffles, growth cones, stereocilia, and synaptosomes, consistent with its roles in dynamic actin-driven membrane protrusions.
• The protein contains an SH3 domain and undergoes phosphorylation and ubiquitination; ubiquitin-mediated degradation is required during G2 phase for cell shape changes associated with cell cycle progression.
• Widely expressed across tissues including brain, heart, lung, liver, kidney, and pancreas, as well as in many epithelial and fibroblastic cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200) of human EPS8, which includes part of the SH3 domain and N-terminal region. This region is relatively conserved across human, mouse, and rat, explaining the cross-reactivity. Consider sequence alignment if working with other species.
• For Western blot, the predicted molecular weight is ~92 kDa (based on UniProt: 91.9 kDa). Bands may be observed near this size; however, phosphorylation or other modifications could cause slight shifts. Validate with appropriate positive and negative controls.
• In ELISA applications, employ well-characterized recombinant EPS8 protein or cell lysates from lines with known expression. Since EPS8 is widely expressed, choose a system with moderate to high expression levels.
• As a polyclonal antibody, lot-to-lot consistency may vary; consider performing pilot experiments when switching lots, particularly for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, supporting diagnostic manufacturers, research laboratories, and institutes from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. For researchers focusing on actin dynamics and cancer biology, this anti-EPS8 polyclonal antibody adds a valuable tool to your repertoire, with CamelBio ensuring reliable supply and technical support throughout your development pipeline.