Anti-EPS15L1 Rabbit Polyclonal Antibody for WB and ELISA - Q9UBC2

Core Product Specifications and Parameters

Biological Background: EPS15L1 Function and Localization

• EPS15L1 (Epidermal growth factor receptor substrate 15-like 1), also known as Eps15R, is a ~94 kDa protein encoded by the EPS15L1 gene in humans.
• It functions as a constitutive component of clathrin-coated pits and is essential for receptor-mediated endocytosis.
• EPS15L1 is involved in the endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR), with ITGB1 internalization requiring DAB2 association.
• The protein localizes to the cell membrane, coated pits, and nucleus, suggesting membrane trafficking and potential nuclear roles.
• Keywords indicate it contains coiled-coil regions, calcium and metal-binding sites, and undergoes phosphorylation and acetylation; alternative splicing generates multiple isoforms.
• EPS15L1 is classified under endocytosis-related proteins and serves as a reference proteome member, highlighting its significance in cell biology research.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 475-754) covers key interaction domains of EPS15L1; consider using this antibody in Western blot with human cell lysates that express the target.
• For ELISA, pair with a suitable detection antibody and validate against recombinant EPS15L1 protein to establish assay conditions.
• Because cross-reactivity is listed for human, verify performance in your specific sample matrix and consider appropriate controls.
• Standard rabbit polyclonal handling (e.g., aliquot storage, brief centrifugation) helps maintain reagent stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive range of IVD raw materials, including validated polyclonal antibodies like this anti-EPS15L1 antibody. From optimized antibody pairs and bulk ancillary reagents to rare target sourcing, we support endocytosis and receptor trafficking research with reliable, scalable supply solutions. Contact us to streamline your procurement from concept to clinic.