Anti-EPS15 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P42566

Core Product Specifications and Parameters

Biological Background: EPS15 Function and Localization

• EPS15 (Epidermal growth factor receptor substrate 15), also known as AF-1p, is a ubiquitously expressed protein involved in the regulation of cell growth and mitogenic signaling.
• It plays a critical role in clathrin-mediated endocytosis as an adaptor for clathrin-coated pit assembly, facilitating the internalization of receptor tyrosine kinases such as EGFR.
• EPS15 is required for post-Golgi trafficking and participates in the maturation of clathrin-coated pits, including invagination and budding.
• It also mediates endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR), with ITGB1 internalization depending on association with DAB2.
• Subcellularly, EPS15 localizes to the cytoplasm, cell membrane, clathrin-coated pits, and early endosome membranes, consistent with its role in endocytic trafficking.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, which may regulate its activity and interactions.
• The EPS15 gene is implicated in chromosomal rearrangements and acts as a proto-oncogene, highlighting its relevance in cancer biology.
• Additionally, EPS15 is involved in host-virus interactions, underscoring its broad biological significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 660-840 of human EPS15, a region likely to be exposed and antigenic in native conditions, making this antibody suitable for detecting endogenous EPS15 in multiple applications.
• For Western blot, the predicted molecular weight is approximately 99 kDa; ensure appropriate gel percentage and transfer conditions.
• In immunocytochemistry/immunofluorescence (IF/ICC), consider fixation and permeabilization conditions to preserve epitope accessibility, as EPS15 localizes to membrane-coated pits.
• Validate cross-reactivity in mouse and rat samples as indicated; perform a titration experiment to determine optimal working dilutions for your specific assay systems.
• Note that polyclonal antibodies may exhibit lot-to-lot variability; it is advisable to include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one-stop provider for IVD raw materials, CamelBio offers high-quality EPS15 antibodies optimized for endocytosis and cell signaling research. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and rare target sourcing to support your diagnostic development from concept to clinic. Partner with us to streamline your procurement and accelerate your assay development workflow.