Anti-EPRS Rabbit Polyclonal Antibody for WB, ELISA - P07814

Core Product Specifications and Parameters

Biological Background: EPRS Function and Localization

• EPRS1 encodes a bifunctional aminoacyl-tRNA synthetase that catalyzes the attachment of L-glutamate and L-proline to their cognate tRNAs, playing a critical role in protein translation.
  Related references:
  PMID:23263184 PMID:24100331 PMID:29576217
• Upon interferon-gamma stimulation, phosphorylated EPRS1 dissociates from the aminoacyl-tRNA synthetase complex and is recruited to form the GAIT complex, which inhibits translation of inflammatory mRNAs via 3'-UTR GAIT elements.
  Related references:
  PMID:15479637 PMID:23071094
• EPRS1 functions downstream of mTORC1 signaling to promote SLC27A1 translocation and long-chain fatty acid uptake in adipocytes, linking it to fat metabolism and lifespan.
  Related references:
  PMID:28178239
• The protein is located in the cytoplasm, cytosol, and membrane, reflecting its diverse functional roles.
• Post-translational modifications include phosphorylation, acetylation, and methylation; it contains ATP-binding, RNA-binding, and zinc-binding domains, underscoring its multifunctional nature.
• Mutations in EPRS1 are associated with leukodystrophy and neurodegeneration.
• It is also known by aliases such as EARS, PARS, QARS, QPRS, HLD15, PIG32, and GLUPRORS.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human cell lines due to the antibody's human cross-reactivity. The predicted molecular weight is ~171 kDa; ensure adequate transfer conditions for high-molecular-weight proteins.
• For ELISA, the synthetic peptide immunogen (amino acids 900-1000 of human EPRS) can serve as a positive control to validate assay conditions.
• The antibody is raised in rabbit and provided as a polyclonal, which may offer broader epitope recognition; validate in the relevant sample system to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials, offering validated antibody products like this anti-EPRS antibody alongside complementary reagents for translation and aminoacyl-tRNA synthetase research. Our supply capabilities encompass monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, supporting diagnostic manufacturers and research labs from discovery to clinical application. Reach out to explore how we can accelerate your project with tailored solutions.