Anti-EPPIN Polyclonal Antibody for WB, ELISA - O95925

Core Product Specifications and Parameters

Biological Background: EPPIN Function and Localization

• EPPIN (Epididymal protease inhibitor), also known as cancer/testis antigen 71 (CT71), is a member of the WAP four-disulfide core domain family (WFDC7) and contains both Kunitz and WAP domains.
• It functions as a serine protease inhibitor critical for male fertility, modulating the hydrolysis of semenogelin-1 (SEMG1) by prostate-specific antigen (PSA/KLK3). EPPIN binds SEMG1, inhibiting sperm motility and providing antimicrobial protection in the ejaculate coagulum.
• EPPIN is localized on the cell surface and secreted, covering the surface of testicular and ejaculated spermatozoa. It is expressed by Sertoli cells in the testis and epithelial cells in the epididymis.
• The protein exhibits alternative splicing, producing isoform 2 which is expressed specifically in the epididymis.
• Key structural features include multiple disulfide bonds, characteristic of the WAP domain, contributing to its stability and function.
• EPPIN possesses antimicrobial activity, protecting spermatozoa within the ejaculate coagulum.
• Tissue-specific expression is predominantly in the testis and epididymis, with weak expression in myoid cells and spermatogenic cells.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to the N-terminal region (aa 22-133) of human EPPIN, which encompasses the Kunitz and WAP domains. This region is conserved across species, consistent with the observed cross-reactivity with mouse and rat.
• For Western blotting, endogenous EPPIN is detected at approximately 15 kDa; consider optimizing sample preparation from testis or epididymis tissue lysates.
• For ELISA applications, validate the antibody in direct or sandwich formats using recombinant EPPIN or biological fluids such as seminal plasma.
• Due to the secreted nature of EPPIN, cell culture supernatants from Sertoli or epididymal cell lines may be suitable sources for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for reproductive health research, including validated antibodies targeting fertility-related proteins like EPPIN. Whether you require bulk quantities of polyclonal antibodies, matched antibody pairs, or ancillary reagents, our one-stop sourcing platform accelerates your assay development from concept to clinic. Contact us for customized solutions and technical support.