Anti-EPO Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P01588

Core Product Specifications and Parameters

Biological Background: Erythropoietin Function and Localization

• Erythropoietin (EPO) is a glycoprotein hormone that regulates erythrocyte proliferation and differentiation, maintaining physiological circulating red blood cell mass. Related references: PMID:28283061
• EPO binds to its receptor EPOR, inducing receptor dimerization and JAK2 activation, which leads to phosphorylation of downstream effectors such as STAT1 and STAT3. Related references: PMID:9774108
• The protein is secreted and circulates in the bloodstream, targeting erythroid progenitor cells in the bone marrow.
• In adults, EPO is mainly produced by the kidney, while the liver is the primary source during fetal and neonatal stages.
• EPO contains conserved cysteine residues that form intramolecular disulfide bonds, critical for its tertiary structure and biological activity.
• Post-translational modifications include extensive N- and O-linked glycosylation, which are essential for in vivo stability and function.
• Genetic variants in EPO are associated with congenital erythrocytosis (ECYT5), a condition characterized by elevated red blood cell mass.
• As a pharmaceutical, recombinant EPO is widely used for treating anemia in chronic kidney disease and chemotherapy patients.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from amino acids 93–193 of human EPO, which may recognize both full-length and mature forms of the protein.
• For Western blotting, consider testing kidney or liver tissue lysates, or plasma samples, as these tissues are primary EPO production sites.
• Immunofluorescence and immunohistochemistry on paraffin-embedded sections may require antigen retrieval; validate in the relevant tissue context.
• ELISA protocols can be optimized for quantitative detection of EPO in serum or cell culture supernatants; cross-reactivity with mouse and rat should be confirmed if switching species.
• Always include appropriate positive and negative controls, and consider batch-to-batch validation for consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For developers working on erythropoietin targets and red blood cell disorders, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our supply chain capabilities ensure that your projects move seamlessly from research to commercialization.