Anti-EPN2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95208

Core Product Specifications and Parameters

Biological Background: EPN2 Function and Localization

• Epsin-2 (EPN2), also known as EPS-15-interacting protein 2, is a member of the epsin family of endocytic adaptor proteins.
• It plays a critical role in clathrin-mediated endocytosis, facilitating the formation of clathrin-coated invaginations and vesicles.
• EPN2 localizes predominantly to the cytoplasm and is specifically associated with clathrin-coated vesicles.
• Tissue expression analysis indicates highest levels in the brain, with lower expression detected in lung and liver.
• The protein undergoes alternative splicing and is subject to post-translational modifications including methylation, phosphorylation, and ubiquitin-like conjugation.
• Keywords associated with EPN2 include endocytosis, lipid-binding, phosphoprotein, and alternative splicing, reflecting its functional versatility in membrane trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 120-300 of human EPN2, a region likely to represent a stable epitope; consider this when designing validation experiments.
• For Western blot applications, note the predicted molecular weight is 68 kDa, but post-translational modifications may cause band shifts; validate under your specific experimental conditions.
• In immunohistochemistry (IHC-P), tissue-specific expression patterns should be considered; brain tissue is expected to yield the strongest signal.
• ELISA performance may benefit from optimizing coating and blocking conditions, using recombinant EPN2 protein as a positive control.
• Cross-reactivity is confirmed for human; for other species, sequence alignment of the immunogen region is recommended prior to testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies such as this anti-EPN2 rabbit polyclonal antibody, essential for research into clathrin-mediated endocytosis and membrane trafficking. Our one-stop sourcing model streamlines your diagnostic development from concept to clinic, offering bulk reagents, antibody pairs, and customized sourcing for even the most specialized targets. Partner with CamelBio to accelerate your project and reduce supply chain complexity.