Anti-Epn2 Rabbit Polyclonal Antibody for WB and ELISA - O95208

Core Product Specifications and Parameters

Biological Background: Epn2/Epsin-2 Function and Localization

• Epsin-2 (also known as EPS-15-interacting protein 2) is encoded by the EPN2 gene in humans and plays a critical role in clathrin-mediated endocytosis.
• It facilitates the formation of clathrin-coated invaginations at the plasma membrane, essential for internalization of receptors and ligands.
• Epsin-2 contains multiple domains including an ENTH (epsin N-terminal homology) domain that binds phosphatidylinositol 4,5-bisphosphate, promoting membrane curvature and recruitment.
• Subcellularly, it localizes to the cytoplasm and is enriched at clathrin-coated vesicles, consistent with its role in endocytosis.
• Post-translational modifications include ubiquitination and phosphorylation, which may regulate its function and interactions.
• Tissue expression is highest in the brain, with lower levels detected in lung and liver, suggesting a prominent role in neuronal endocytosis.
• Alternative splicing generates multiple isoforms, potentially modulating its activity and binding partners.
• Additional keywords such as lipid-binding, phosphoprotein, methylation, and Ubl conjugation highlight its regulatory complexity.

Experimental Guidance and Technical Tips

• For Western blotting, a recommended starting dilution is 1:500-1:2000; however, optimal dilution should be determined empirically using your specific lysates and detection system.
• The antibody is expected to detect a band at approximately 68 kDa in human, mouse, and rat samples, with the strongest signal in brain tissue.
• For ELISA, the immunizing peptide can serve as a positive control; consider coating with peptide and detecting antibody binding via standard procedures.
• As a polyclonal antibody, it may recognize multiple isoforms; confirm specificity by using knockout or knockdown controls if available.
• To avoid non-specific binding, consider blocking with 5% non-fat milk or BSA and including appropriate secondary antibody controls.
• Since Epn2 is involved in membrane trafficking, subcellular fractionation may enhance detection in vesicle-enriched samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing a broad portfolio of high-quality IVD antibodies, including this anti-Epn2 polyclonal antibody. Our comprehensive offering covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents for endocytosis and cell membrane trafficking studies. Let CamelBio streamline your raw material sourcing from discovery to clinical application.