Anti-EPN1 (Epsin-1) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y6I3

Core Product Specifications and Parameters

Biological Background: EPN1 Function and Localization

• Epsin-1 (EPN1), also known as EH domain-binding mitotic phosphoprotein and EPS-15-interacting protein 1, is encoded by the EPN1 gene (GeneID: 29924) and serves as a key adaptor in clathrin-mediated endocytosis.
• The protein binds specifically to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), inducing curvature and promoting the assembly of clathrin-coated invaginations (By similarity).
• EPN1 directly regulates receptor-mediated endocytosis, a process essential for nutrient uptake, signal attenuation, and membrane recycling. Related references: PMID:10393179 PMID:10557078
• Subcellularly, EPN1 localizes to the cytoplasm, cell membrane, nucleus, and specifically concentrates at clathrin-coated pits, consistent with its endocytic role.
• The protein is subject to multiple post-translational modifications, including phosphorylation, methylation, and Ubl conjugation, which likely modulate its function and interaction network.
• EPN1 contains an amino-terminal ENTH domain, central EH domain-binding motifs, and undergoes alternative splicing to produce isoforms that may diversify its functional roles.

Experimental Guidance and Technical Tips

• The immunogen (residues 130–180) lies within a region that may include functionally important motifs; consider mapping its conservation in mouse and rat to explain the observed cross-reactivity.
• For Western blot, a band near 60 kDa is expected; validate detection in the relevant sample system (human, mouse, or rat) and include appropriate loading controls.
• In IHC-P, antigen retrieval and endogenous peroxidase blocking are recommended; titrate the antibody and include a known positive control tissue to confirm specificity.
• Polyclonal antibodies may exhibit lot-to-lot variability; running a positive control lysate with each new lot is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with one-stop access to IVD raw materials. This anti-EPN1 polyclonal antibody meets the needs of cell biology and endocytosis research, and we can additionally source validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and even rare-target raw materials. Let us streamline your procurement from discovery to clinical application.