Anti-EPM2A Rabbit Polyclonal Antibody for WB, ELISA - O95278

Core Product Specifications and Parameters

Biological Background: Laforin Function and Localization

• Laforin is a dual-specificity phosphatase encoded by the EPM2A gene, also known as glucan phosphatase or glycogen phosphatase. It plays a critical role in glycogen metabolism by dephosphorylating glycogen, thus preventing hyperphosphorylation and the formation of insoluble aggregates, which are hallmarks of Lafora disease. Related references: PMID:16901901, PMID:23922729, PMID:25538239
• Laforin exhibits strong phosphatase activity towards complex carbohydrates in vitro and can also dephosphorylate phosphotyrosine and synthetic substrates like pNPP, but shows low activity towards phosphoserine/phosphothreonine. Related references: PMID:11001928, PMID:11220751, PMID:11739371
• It forms a complex with NHLRC1/malin and HSP70, which targets misfolded proteins for degradation via the ubiquitin-proteasome system, and also serves as a scaffold to promote PPP1R3C/PTG ubiquitination. Related references: PMID:23922729
• Additionally, laforin promotes proteasome-independent protein degradation through the macroautophagy pathway. Related references: PMID:20453062
• The protein exists in multiple isoforms; isoform 2 lacks phosphatase activity and may act as a dominant-negative regulator of isoform 1 and isoform 7. Related references: PMID:18617530, PMID:22036712
• Laforin is widely expressed in heart, skeletal muscle, kidney, pancreas, and brain, with isoform 4 also expressed in placenta.
• Subcellularly, laforin localizes to the cytoplasm, endoplasmic reticulum membrane, cell membrane, and nucleus.
• Mutations in EPM2A cause Lafora disease, a progressive myoclonus epilepsy with glycogen inclusion bodies (Lafora bodies).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 244–331, a region present in full-length laforin. This polyclonal antibody is likely to detect multiple isoforms that include this epitope.
• For Western blot, the expected molecular weight is approximately 37 kDa. Validate band specificity using positive controls such as human brain or skeletal muscle lysates, and consider pre-adsorption or peptide blocking to confirm signal.
• For ELISA, the recombinant immunogen can serve as a capture antigen or positive control. Ensure proper coating and detection conditions are optimized.
• Cross-reactivity with mouse and rat has been reported, but users should verify performance in their specific sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets such as EPM2A/laforin implicated in neurological and metabolic disorders, we specialize in sourcing rare-target raw materials to accelerate your assay development. Our integrated services help streamline your workflow from concept to clinic.