Anti-EPHX2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P34913

Core Product Specifications and Parameters

Biological Background: EPHX2 Function and Localization

• EPHX2 is a bifunctional enzyme: its C-terminal domain has epoxide hydrolase activity (PubMed:12574510, PubMed:12869654, PubMed:22798687), and its N-terminal domain has lipid phosphatase activity (PubMed:12574510, PubMed:22217705, PubMed:22387545). Related references: PMID:12574510, PMID:12869654, PMID:22798687, PMID:22217705, PMID:22387545
• The epoxide hydrolase domain degrades potentially toxic epoxides, contributing to xenobiotic metabolism, and regulates steady-state levels of physiological mediators (PubMed:12574510, PubMed:12869654, PubMed:21217101). Related references: PMID:12574510, PMID:12869654, PMID:21217101
• The lipid phosphatase domain shows highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, with lower activity towards lysolipids of sphingolipid and isoprenoid phosphates (PubMed:12574510, PubMed:22217705, PubMed:22387545). Related references: PMID:12574510, PMID:22217705, PMID:22387545
• EPHX2 localizes to the cytoplasm and peroxisome.
• Post-translational modifications include acetylation and phosphorylation (UniProt keywords).
• Alternative splicing produces multiple isoforms of EPHX2.

Experimental Guidance and Technical Tips

• The immunogen (aa 30-240) lies within the N-terminal lipid phosphatase domain; for assays requiring detection of the C-terminal epoxide hydrolase domain, consider using antibodies raised against other regions.
• For Western blotting, a band around 63 kDa is expected; validate in the relevant sample system.
• EPHX2 is cytoplasmic and peroxisomal; for immunofluorescence, appropriate permeabilization may be needed.
• The antibody is suitable for ELISA; include appropriate controls and validate sensitivity and specificity in your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focused on EPHX2-related pathways in xenobiotic metabolism and lipid signaling, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your IVD development needs.