Anti-EPHX2 Rabbit Monoclonal Antibody for WB and ELISA - P34913

Core Product Specifications and Parameters

Biological Background: EPHX2 Function and Localization

• EPHX2 is a bifunctional enzyme, featuring an N-terminal lipid phosphatase domain and a C-terminal epoxide hydrolase domain. Related references: PMID:12574510
• The C-terminal domain exhibits epoxide hydrolase activity, metabolizing epoxides and arene oxides, which is crucial for xenobiotic detoxification and the regulation of physiological lipid mediators. Related references: PMID:12574510, PMID:12869654, PMID:22798687, PMID:21217101
• The N-terminal domain functions as a lipid phosphatase, with activity towards specific phosphorylated lipids and lyso-glycerophospholipids, and also has some activity on lysolipids of sphingolipid and isoprenoid phosphates. Related references: PMID:12574510, PMID:22217705, PMID:22387545
• EPHX2 localizes to the cytoplasm and peroxisomes.
• EPHX2 undergoes alternative splicing, producing multiple isoforms.
• Key post-translational modifications include acetylation and phosphorylation; the enzyme requires magnesium as a cofactor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminus (aa 1-100) of human EPHX2, which may help ensure specificity for the mature protein or certain isoforms.
• For western blotting, the predicted molecular weight is 63 kDa under denaturing conditions; use appropriate gel percentage and transfer conditions.
• Cross-reactivity with mouse and rat enables multispecies analysis; validate performance in the relevant sample system.
• This antibody is suitable for ELISA; consider optimization of coating and detection protocols for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to antibodies, antigens, and ancillary reagents. For studies on lipid metabolism, xenobiotic detoxification, and epoxide hydrolase pathways, this high-quality anti-EPHX2 monoclonal antibody exemplifies our commitment to supplying validated tools. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk reagents, and rare target raw-material sourcing to support your entire development workflow.