Anti-EPHX1 Rabbit Polyclonal Antibody for WB, ELISA - P07099

Core Product Specifications and Parameters

Biological Background: EPHX1 Function and Localization

• EPHX1 is a biotransformation enzyme that hydrolyzes arene and aliphatic epoxides to less reactive and more water-soluble dihydrodiols, playing a central role in detoxification pathways.
• The enzyme participates in the metabolism of endogenous lipids, including epoxide-containing fatty acids, thereby contributing to the regulation of lipid metabolism and cellular signaling. Related references: PMID:22798687
• EPHX1 metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid and glycerol, implicating it in endocannabinoid system modulation. Related references: PMID:24958911
• Subcellularly, EPHX1 is anchored to microsomal and endoplasmic reticulum membranes, consistent with its role in xenobiotic and lipid processing.
• The protein is predominantly expressed in the liver, aligning with its critical function in hepatic detoxification.
• Post-translational modifications include methylation, and its native structure has been verified by direct protein sequencing.
• Additional commonly used aliases for EPHX1 include MEH, EPHX, EPOX, and HYL1.

Experimental Guidance and Technical Tips

• For Western blotting, expect a primary band at approximately 53 kDa; as a polyclonal antibody, it may detect additional isoforms or processing products—validate in the relevant sample system.
• When using ELISA, optimize the antibody coating and detection conditions; consider pairing with an appropriate enzyme-labeled secondary antibody for your assay format.
• The immunogen represents a large N-terminal fragment (aa 20–320), suggesting potential recognition of both native and denatured EPHX1; pilot experiments should determine the optimal working dilution for each application.
• Given the validated cross-reactivity with mouse, this antibody is suitable for comparative studies using human and mouse tissues, but independent verification in the specific experimental context is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering a comprehensive range of high-quality antibodies and reagents to support your diagnostic development from concept to clinic. With expertise in validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing, we can address the diverse needs of metabolism and detoxification research. Our EPHX1 antibody exemplifies our commitment to supplying reliable tools that empower your next breakthrough.