Anti-Ephrin B2 Rabbit Polyclonal Antibody for WB, ELISA - P52799

Core Product Specifications and Parameters

Biological Background: Ephrin B2 Function and Localization

• Ephrin-B2 (EFNB2) is a cell surface transmembrane ligand for Eph receptor tyrosine kinases, critical for contact-dependent bidirectional signaling in neuronal, vascular, and epithelial development.
• It plays a central role in heart morphogenesis and angiogenesis by regulating cell adhesion and migration, particularly through EPHB4-mediated forward signaling that controls cellular repulsion.
• Ephrin-B2 binds promiscuously to Eph receptors including EPHA4, EPHA3, and EPHB4, triggering both forward (receptor) and reverse (ligand) signaling pathways.
• It also functions as a receptor for Hendra and Nipah viruses, mediating viral entry during infection.
• Subcellularly, ephrin-B2 localizes to the cell membrane and cell junctions, specifically adherens junctions, enabling cell-cell communication.
• Expressed predominantly in lung and kidney tissues, it is involved in developmental processes, neurogenesis, and angiogenesis.
• The protein undergoes post-translational modifications such as glycosylation, disulfide bond formation, and phosphorylation, which regulate its activity.
• UniProt keywords highlight its roles in differentiation, host-virus interaction, and transmembrane signaling (3D-structure, Angiogenesis, Cell junction, Cell membrane, Developmental protein, Differentiation, Disulfide bond, Glycoprotein, Host cell receptor for virus entry, Host-virus interaction, Membrane, Methylation, Neurogenesis, Phosphoprotein, Proteomics identification, Receptor, Reference proteome, Signal, Transmembrane, Transmembrane helix).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 28-229) of human ephrin-B2, which may detect both the full-length and potential soluble forms in WB.
• For Western blot, consider using reducing conditions to resolve the 37 kDa band; validate specificity with appropriate controls given reported endogenous expression in lung and kidney tissues.
• The antibody shows cross-reactivity with mouse and rat, making it suitable for translational studies in these model organisms; however, confirm reactivity in your target species and tissue type.
• ELISA applications may be developed using recombinant ephrin-B2 protein; optimize coating and detection conditions based on the polyclonal nature of the antibody.
• Given the role of ephrin-B2 in angiogenesis and neuronal development, consider functional studies in cell migration or repulsion assays to complement detection applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to diagnostic manufacturers and research laboratories worldwide. This ephrin-B2 antibody supports cardiovascular and neurobiology research applications, and CamelBio ensures reliable access to a complete portfolio of reagents for related targets such as Eph receptors and other ephrin family members. Whether you require custom antibody development or bulk supply, CamelBio bridges your concepts to clinical readiness.