Anti-Ephrin B2 Monoclonal Antibody for WB, IHC-P, ELISA - P52799

Core Product Specifications and Parameters

Biological Background: Ephrin B2 (EFNB2) Function and Localization

• Ephrin-B2 (also known as LERK-5, HTK-L) is a single-pass type I membrane protein encoded by the EFNB2 gene. It belongs to the ephrin family of receptor tyrosine kinase ligands.
• As a key developmental regulator, it mediates bidirectional cell-cell signaling upon binding to Eph receptors (e.g., EPHA4, EPHA3, EPHB4), controlling cell migration, repulsion, and adhesion essential for neuronal, vascular, and epithelial patterning.
• The EPHB4-EFNB2 ligand-receptor pair plays a central role in heart morphogenesis and angiogenesis by regulating endothelial cell sorting and vessel sprouting.
• Ephrin-B2 is localized at the cell membrane and concentrates at adherens junctions, ensuring juxtacrine signaling.
• It is predominantly expressed in lung and kidney, reflecting its functional involvement in epithelial morphogenesis and vascular networks in these organs.
• Post-translational modifications include glycosylation, phosphorylation, and methylation, which modulate its signaling activity.
• Additionally, Ephrin-B2 serves as a cellular receptor for Hendra and Nipah viruses, facilitating viral entry.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular domain (aa 1-100), which includes the receptor-binding region; this design detects both full-length and proteolytic fragments.
• For Western blot, a band at ~37 kDa is expected under reducing conditions; validate in your specific sample type, as post-translational modifications may alter migration.
• IHC-P may require antigen retrieval; consider testing on human lung or kidney sections, where EFNB2 expression is high, and include appropriate controls.
• ELISA: The antibody can be used for antigen capture or detection; optimize coating buffer and incubation conditions for your analyte.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials, covering every stage from concept to clinic. This Ephrin B2 monoclonal antibody supports research on angiogenesis, neurodevelopment, and viral entry mechanisms. Explore our inventory of validated antibody pairs, bulk ancillary reagents, and custom sourcing services to streamline your assay development.