Anti-Ephrin A1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P20827

Core Product Specifications and Parameters

Biological Background: Ephrin A1 Function and Localization

• Ephrin-A1 is a GPI-anchored cell surface ligand that binds to Eph receptor tyrosine kinases, mediating contact-dependent bidirectional signaling crucial for cell migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. Related references: PMID:17332925, PMID:18794797
• It plays a key role in angiogenesis by regulating vascular endothelial cell differentiation and migration through activation of EPHA2.
• Acts as a ligand for EPHA3, inhibiting epithelial-to-mesenchymal transition of cardiac cells and contributing to heart development. Related references: PMID:12794130
• May also contribute to dendritic spine morphogenesis (by similarity).
• Subcellular localization: anchored to the cell membrane via a GPI moiety; also found as a secreted form.
• Tissue specificity: primarily expressed in brain, down-regulated in primary glioma tissues, with soluble monomeric form detected in glioblastoma multiforme and breast cancer cells.
• Post-translational modifications include glycosylation, GPI-anchor attachment, and disulfide bond formation; classified as a lipoprotein and a potential tumor suppressor.

Experimental Guidance and Technical Tips

• The immunization peptide (amino acids 1–100) targets the N-terminal extracellular region, which may improve recognition of both cell surface and secreted ephrin-A1 forms. Consider this when designing capture or detection assays.
• In Western blot (WB), the predicted protein weight is ~24 kDa. Due to glycosylation and GPI-anchor processing, the actual band may appear at a higher molecular weight. Validate in relevant human or mouse tissue/cell lysates.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), the membrane and secreted nature of ephrin-A1 may require optimized fixation and permeabilization conditions to preserve antigenicity. Test with known positive controls (e.g., brain tissue, endothelial cells).
• In ELISA, this monoclonal antibody can serve as a capture or detection reagent. Pair with another clone or a polyclonal antibody targeting a distinct epitope for sandwich ELISA development.
• Species cross-reactivity has been tested for human and mouse (as indicated). For other species, consult sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, from validated antibodies and antigen pairs to custom reagent sourcing. This anti-Ephrin A1 monoclonal antibody is part of our expanding catalog for angiogenesis and oncology research tools. Whether you need bulk supplied antibodies, conjugate-ready reagents, or help sourcing rare-target biologicals, CamelBio streamlines your supply chain from R&D to commercial scale.