Anti-EPHB4 Rabbit Polyclonal Antibody for WB, ELISA - P54760

Core Product Specifications and Parameters

Biological Background: EPHB4 Function and Localization

• Encodes a receptor tyrosine kinase that binds transmembrane ephrin-B ligands, mediating contact-dependent bidirectional signaling (forward and reverse signaling) between adjacent cells.
• Together with its cognate ligand EFNB2, regulates cell adhesion, migration, and repulsion, playing essential roles in heart morphogenesis, angiogenesis, and blood vessel remodeling.
• Phosphorylates inactive tyrosine-protein kinase EPHB6, promoting its interaction with SH2 domain-containing signaling adapters. Related references: PMID:34431498
• Localized to the cell membrane, consistent with its function as a transmembrane receptor.
• Abundantly expressed in placenta, with detectable levels in kidney, liver, lung, pancreas, skeletal muscle, heart, and certain hematopoietic cells.
• Associated with angiogenesis, developmental processes, and disease variants (as indicated by keywords such as 'Disease variant' and 'Developmental protein').

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–540, a region encompassing part of the extracellular domain, which may contribute to specificity for native EPHB4. When using this antibody, consider validating recognition of the target in the relevant sample system.
• For WB applications, prepare lysates from cell lines or tissues known to express EPHB4 (e.g., placenta, kidney, or liver) and expect a band near 108 kDa. Include appropriate controls to confirm specificity.
• For ELISA, the antibody can be explored as a capture or detection reagent; pairing with an antibody targeting a non-overlapping epitope may improve sandwich assay performance.
• Given cross-reactivity with mouse, validate the antibody in mouse-derived samples if intended for such models; note that the immunogen design is based on human sequence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement pathway for research and diagnostic raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For studies targeting EPHB4’s role in angiogenesis and cardiovascular development, we can support your sourcing needs for rare targets and companion reagents. Our team assists diagnostic manufacturers and laboratories with technical services and consulting, from concept to clinic.