Anti-EPHA5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P54756

Core Product Specifications and Parameters

Biological Background: EPHA5 Function and Localization

• Ephrin type-A receptor 5 (EPHA5), also known as EHK-1, EK7, or TYRO4, is a member of the ephrin receptor subfamily of receptor tyrosine kinases that bind GPI-anchored ephrin-A ligands, with EFNA5 likely serving as its primary cognate ligand.
• Upon ligand binding, EPHA5 mediates contact-dependent bidirectional signaling: forward signaling through the receptor and reverse signaling through the ephrin ligand, orchestrating critical developmental processes.
• EPHA5 functions as an axon guidance molecule, essential for the formation of the retinotectal, entorhino-hippocampal, and hippocamposeptal pathways during nervous system development.
• In the adult brain, EPHA5 contributes to synaptic plasticity by regulating synaptogenesis, highlighting its role beyond embryonic development.
• Beyond the nervous system, EPHA5 and EFNA5 interaction mediates communication between pancreatic islet cells, regulating glucose-stimulated insulin secretion (By similarity).
• Subcellularly, EPHA5 localizes to the cell membrane and is enriched in cell projections such as axons and dendrites, consistent with its role in neuronal connectivity.
• The protein is almost exclusively expressed in the nervous system, notably in cortical neurons, cerebellar Purkinje cells, and pyramidal neurons, with an increasing expression gradient from forebrain to hindbrain and spinal cord.
• EPHA5 undergoes alternative splicing and post-translational modifications including glycosylation and phosphorylation, features common to receptor tyrosine kinases that modulate signaling activity.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (amino acids 1-130) of human EPHA5, which includes the signal peptide and part of the ephrin ligand-binding domain; this design may provide specificity for the extracellular portion of the receptor.
• For Western blotting, the predicted molecular weight is approximately 115 kDa; due to glycosylation, the observed band may appear slightly higher. Consider using positive control lysates from brain tissue or neuronal cell lines (e.g., SH-SY5Y) known to express EPHA5.
• In immunofluorescence/immunocytochemistry (IF/ICC), EPHA5 is expected to localize at the cell membrane and within neuronal projections such as axons and dendrites. Validate staining patterns in cultured primary neurons or neuronal cell lines, and include appropriate controls to confirm specificity.
• ELISA applications may be used for detecting EPHA5 in cell lysates or tissue homogenates; however, optimization of coating and detection conditions is recommended based on the sample matrix.
• Given cross-reactivity with mouse and rat, this antibody is suitable for studies in rodent models, but confirm expression levels in the target tissue, as EPHA5 exhibits region-specific gradients in the nervous system.

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CamelBio serves as a one-stop sourcing partner for diagnostic manufacturers, research laboratories, and institutes, offering comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. This EPHA5 polyclonal antibody supports neuroscience research on axon guidance, synaptic plasticity, and receptor tyrosine kinase signaling, aligning with our commitment to streamline your procurement process.