Anti-EPHA1 Rabbit Polyclonal Antibody for WB, ELISA - P21709

Core Product Specifications and Parameters

Biological Background: EPHA1 Function and Localization

• EPHA1 is a receptor tyrosine kinase belonging to the ephrin receptor subfamily, also known as EPH tyrosine kinase, EPH tyrosine kinase 1, Erythropoietin-producing hepatoma receptor, and Tyrosine-protein kinase receptor EPH.
• Functions as a receptor for membrane-bound ephrin-A ligands, mediating contact-dependent bidirectional signaling; forward signaling through the receptor and reverse signaling through the ephrin.
• Upon activation by EFNA1, EPHA1 induces cell attachment to the extracellular matrix, inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC.
• Plays a role in angiogenesis and regulates cell proliferation; may also be involved in apoptosis.
• Subcellular localization: primarily located at the cell membrane.
• Tissue specificity: Overexpressed in several carcinomas, indicating a potential role in cancer.
• Keywords highlight features including Alternative splicing, ATP-binding, Glycoprotein, Kinase, Phosphoprotein, Ubl conjugation, and Transmembrane regions, suggesting multiple regulatory mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 1-230), which may recognize full-length as well as potential soluble isoforms or shed ectodomains; validating band sizes in relevant cell lysates is recommended.
• For Western blot, the predicted molecular weight is 108kDa; consider using appropriate running and transfer conditions for high molecular weight proteins.
• In ELISA, the antibody can be used for detection of recombinant or endogenous EPHA1; validate in the relevant sample system.
• Cross-reactivity with mouse EPHA1 suggests it may be suitable for mouse model studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner for a validated rabbit polyclonal antibody targeting EPHA1, ideal for cancer and angiogenesis research. We support your assay development and manufacturing needs with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our supply capacity extends to rare target raw-material sourcing, ensuring comprehensive support from concept to clinic.