Anti-EPCR/CD201 Monoclonal Antibody for WB, ELISA - Q64695

Core Product Specifications and Parameters

Biological Background: EPCR/CD201 Function and Localization

• EPCR (Endothelial protein C receptor), also known as activated protein C receptor, centrocyclin, and centrosomal protein CCD41, is encoded by the Procr gene.
• Binds activated protein C and enhances protein C activation by the thrombin-thrombomodulin complex, playing a central role in the protein C pathway that controls blood coagulation.
• Subcellular localization: Membrane; single-pass type I transmembrane protein.
• Tissue specificity: Expressed in endothelial cells.
• Contains characteristic structural features including a disulfide bond, glycosylation sites, a signal peptide, and a transmembrane helix.
• Classified under blood coagulation and hemostasis pathways; essential for the anticoagulant protein C system.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (residues 18-210) of mouse EPCR, suggesting the antibody likely recognizes an epitope in this region; consider epitope mapping for domain-specific applications.
• For Western blotting, the predicted protein weight is approximately 27 kDa; ensure appropriate gel percentage and transfer conditions to resolve this molecular weight.
• ELISA applications may benefit from using the immunogen or recombinant EPCR as a positive control; validate linearity and sensitivity with dilution series.
• Confirm antibody specificity using positive controls (e.g., endothelial cell lysates) and negative controls (e.g., tissues or cells lacking EPCR expression).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials like this anti-EPCR/CD201 monoclonal antibody. Our extensive catalog supports hemostasis and coagulation research with validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Count on CamelBio for reliable sourcing of rare-target raw materials, helping you move efficiently from concept to clinic.