Anti-EpCAM Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P16422

Core Product Specifications and Parameters

Biological Background: EpCAM Function and Localization

• EpCAM (Epithelial cell adhesion molecule), also known as TROP1, EGP40, and CD326, is a transmembrane glycoprotein encoded by the EPCAM gene.
• Functions as a homophilic cell adhesion molecule, mediating interactions between intestinal epithelial cells and intraepithelial lymphocytes, contributing to the mucosal immunological barrier.
• Plays a role in embryonic stem cell proliferation and differentiation; up-regulates the expression of FABP5, MYC, and cyclins A and E.
• Localizes to the lateral cell membrane and tight junctions, contributing to cell-cell adhesion and junctional integrity.
• Highly and selectively expressed on undifferentiated embryonic stem cells, with levels rapidly diminishing upon differentiation.
• Expressed in almost all epithelial cell membranes, but absent from mesodermal and neural cell membranes; prominently found on the surface of adenocarcinomas.
• Implicated in hereditary nonpolyposis colorectal cancer (Lynch syndrome) and recognized as a tumor antigen; contains a signal peptide and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1–100) of human EpCAM, encompassing a region critical for homophilic interactions.
• In Western blotting (WB), a band of approximately 35 kDa is expected; however, native glycosylated EpCAM may migrate at a higher molecular weight. Validate detection in epithelial cell lines (e.g., MCF-7, HepG2) which express EpCAM endogenously.
• For immunohistochemistry-paraffin (IHC-P), EpCAM is strongly expressed on the membrane of epithelial cells. Consider appropriate antigen retrieval methods and include positive (e.g., colon carcinoma) and negative (e.g., mesenchymal tissue) controls.
• In ELISA applications, this monoclonal antibody can be tested as a capture or detection reagent for quantifying soluble or cell-surface EpCAM. Cross-reactivity with mouse and rat EpCAM may facilitate analysis across species; however, epitope conservation should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your reliable one-stop sourcing partner, offering comprehensive raw material solutions for epithelial cell adhesion and cancer research applications. From validated antibody pairs and recombinant proteins to ancillary reagents, we support your IVD development pipeline with scalable, research-grade products. This anti-EpCAM monoclonal antibody exemplifies our commitment to delivering rare and validated target reagents that accelerate your transition from bench to clinical testing.