Anti-Epcam Polyclonal Antibody for WB, ELISA - Q99JW5

Core Product Specifications and Parameters

Biological Background: Epcam Function and Localization

• Epcam (Epithelial cell adhesion molecule), also known as EGP314, CD326, TROP1, and Tacsd1, is a type I transmembrane glycoprotein involved in cell-cell adhesion.
• It contains an N-terminal signal peptide, an extracellular domain with thyroglobulin type-1-like repeats, a single transmembrane helix, and a short cytoplasmic tail.
• Epcam localizes to the lateral cell membrane and tight junctions in epithelial cells, where it mediates homophilic interactions between intestinal epithelial cells and intraepithelial lymphocytes, contributing to the mucosal immunological barrier.
• The protein plays a role in embryonic stem cell proliferation and differentiation, and it up-regulates the expression of FABP5, MYC, and cyclins A and E.
• It is a disulfide-linked glycoprotein, and its expression is associated with various cancers, often referred to as Tumor-associated calcium signal transducer 1.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 24-266) of mouse Epcam, which may generate antibodies recognizing multiple epitopes across the native and denatured protein.
• For Western blot (WB), consider using whole-cell lysates from epithelial tissues or cell lines (e.g., mouse colon or human Caco-2 cells), and validate the band at the expected molecular weight of ~35 kDa.
• In ELISA applications, the antibody may be used to capture or detect Epcam in solution; however, optimal coating concentrations and blocking conditions should be empirically determined.
• Given the reported cross-reactivity with human and rat, this antibody may be suitable for comparative studies, but individual species validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

As your dedicated IVD raw-material provider, CamelBio offers a comprehensive selection of antibodies, including this anti-Epcam polyclonal antibody, to support epithelial cell adhesion and cancer biomarker research. Beyond single reagents, we supply validated antibody pairs, bulk ancillary materials, and custom sourcing solutions for rare targets, helping diagnostic manufacturers accelerate from concept to clinic.