Anti-EPB42 Polyclonal Antibody for WB, ELISA - P16452

Core Product Specifications and Parameters

Biological Background: EPB42 Function and Localization

• EPB42 encodes Protein 4.2, also known as erythrocyte membrane protein band 4.2, a critical component of the erythrocyte membrane skeleton.
• It functions as part of the ankyrin-1 complex, which is essential for maintaining the stability and biconcave shape of red blood cells.
• Subcellularly localized to the cell membrane, cytoplasm, and cytoskeleton, consistent with its role in linking the membrane to the underlying spectrin-actin network.
• Post-translational modifications include myristoylation and phosphorylation, which may regulate membrane association and protein interactions.
• Mutations in EPB42 are associated with hereditary hemolytic anemia, underscoring its clinical significance in erythrocyte disorders.
• Alternative splicing generates multiple isoforms, potentially diversifying its functional repertoire.
• Classified as a lipoprotein, suggesting association with lipid-rich membrane microdomains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 10-240 of human EPB42; use this sequence for cross-species alignment to predict reactivity with non-human samples.
• For Western blot, expect a band at approximately 77 kDa; validate specificity using positive controls such as human erythrocyte lysates or recombinant protein.
• In ELISA applications, optimize coating antigen concentration and antibody titer; consider pairing with a matched detection antibody for sandwich ELISA development.
• Cross-reactivity with mouse has been reported; verify performance in your specific mouse model or tissue type prior to large-scale experiments.
• As a polyclonal antibody, batch-to-batch consistency should be confirmed using reference standards for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated B2B partner for IVD raw material sourcing, supporting hematology and membrane biology research with high-quality antibodies and reagents. We provide validated polyclonal and monoclonal antibodies, bulk ancillary supplies, and specialized sourcing for rare erythrocyte targets like EPB42. Accelerate your diagnostic development with our comprehensive portfolio and technical consultation services.