Anti-EPB42 (Erythrocyte Protein 4.2) Rabbit Polyclonal Antibody for IF/ICC and ELISA - P16452

Core Product Specifications and Parameters

Biological Background: EPB42 (Erythrocyte Protein 4.2) Function and Localization

• EPB42 encodes erythrocyte membrane protein band 4.2 (protein 4.2), a critical component of the red blood cell membrane skeleton.
• It functions as a subunit of the ankyrin-1 complex, which is essential for maintaining erythrocyte membrane stability and normal cell shape.
• Alternative splicing produces multiple isoforms that may contribute to functional diversity during erythrocyte maturation.
• Protein 4.2 localizes to the cell membrane, cytoplasm, and cytoskeleton in erythrocytes.
• Post-translational modifications include myristoylation and phosphorylation, which likely regulate membrane association and protein activity.
• Mutations in EPB42 are associated with hereditary hemolytic anemia, underscoring its importance in red cell integrity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal region (amino acids 589–691) of human EPB42, which is conserved across isoforms; consider this in isoform‑specific studies.
• For IF/ICC, note that EPB42 distributes at the cell membrane and cytoskeleton; ensure proper permeabilization to access intracellular epitopes.
• In ELISA applications, the antibody can serve as a capture or detection reagent; validate pairing and working concentrations in erythrocyte lysates or relevant biological samples.
• Cross‑reactivity with mouse EPB42 makes this antibody suitable for translational studies in mouse models; validate performance in the target tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti‑EPB42 polyclonal antibody supports research into erythrocyte membrane disorders and anemia diagnostics. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare target sourcing, CamelBio streamlines your procurement workflow.