Anti-EPB41L2 Polyclonal Antibody for WB, IF/ICC, ELISA - O43491

Core Product Specifications and Parameters

Biological Background: EPB41L2 Function and Localization

• EPB41L2 encodes Band 4.1-like protein 2, also known as Generally expressed protein 4.1 (4.1G) and Erythrocyte membrane protein band 4.1-like 2, a member of the protein 4.1 family that links the cytoskeleton to the plasma membrane.
• EPB41L2 is required for the recruitment of the dynein-dynactin complex and NUMA1 to the mitotic cell cortex during anaphase, ensuring proper spindle orientation and chromosome segregation. Related references: PMID:23870127
• The protein localizes to the cytoplasm, cytoskeleton, cell cortex, and cell membrane, consistent with its role in cortical cytoskeletal organization and membrane attachment.
• EPB41L2 is widely expressed across tissues, suggesting a general function in cytoskeletal architecture and cell division.
• The gene undergoes alternative splicing to produce multiple isoforms, and the protein is subject to acetylation, phosphorylation, and ubiquitination, as revealed by proteomics and direct sequencing studies.
• It contains an actin-binding domain and participates in cell cycle regulation, particularly during mitosis, with additional involvement in cellular transport processes.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-210), so the antibody may react with multiple isoforms sharing this domain; consider verifying specificity in your sample system.
• For WB, reduce and denature samples thoroughly; the predicted molecular weight is approximately 113 kDa, but post-translational modifications or isoforms may shift the band.
• In IF/ICC, because EPB41L2 localizes to the cell cortex and cytoskeleton, permeabilization with Triton X-100 or similar detergents is typically necessary; fixatives such as PFA are suitable.
• For ELISA, the polyclonal nature allows use as both capture and detection reagent; validate performance with recombinant protein or cell lysates in your buffer conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying IVD manufacturers, research institutes, and clinical laboratories with a comprehensive range of raw materials and technical support for cytoskeletal and cell division research. In addition to this EPB41L2 polyclonal antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets—all under one procurement channel. Let our team streamline your raw-material acquisition so you can focus on developing robust diagnostic assays.