Anti-EPB41 Rabbit Polyclonal Antibody for WB, ELISA - P11171

Core Product Specifications and Parameters

Biological Background: Protein 4.1 Function and Localization

• Protein 4.1 (EPB41), also known as 4.1R or Band 4.1, is a major structural component of the erythrocyte membrane skeleton, encoded by the EPB41 gene in humans.
• It stabilizes the spectrin-actin network, thereby regulating membrane mechanical stability and deformability in red blood cells.
• Protein 4.1 recruits DLG1 to membranes, contributing to cell polarity and signaling.
• During mitosis, it is required for the recruitment of the dynein-dynactin complex and NUMA1 to the mitotic cell cortex, ensuring proper chromosome segregation. Related references: PMID:23870127
• Subcellularly, it is localized to the cytoplasm, cytoskeleton, cell cortex, and nucleus, reflecting its diverse roles.
• Post-translational modifications include phosphorylation and glycosylation, as indicated by the keywords.
• Additional keywords: alternative splicing, calmodulin-binding, and involvement in hereditary elliptocytosis and pyropoikilocytosis.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 210-500; consider verifying reactivity against full-length protein and isoforms in your sample system.
• For western blotting, the predicted molecular weight is approximately 97 kDa; however, alternative splicing may produce additional bands. Optimize blocking and antibody dilution accordingly.
• In ELISA applications, include appropriate controls to confirm specificity, especially when using complex sample types.
• Cross-reactivity is reported for human, mouse, and rat; validate detection in the relevant species before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a reliable supply of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-EPB41 antibody. Supporting membrane skeleton research and cell division studies, we offer bulk orders, ancillary reagents, and customized sourcing for rare targets to streamline your assay development from concept to clinic.