Anti-EPB41 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11171

Core Product Specifications and Parameters

Biological Background: EPB41 Function and Localization

• EPB41 encodes Protein 4.1, also known as 4.1R, Band 4.1, or EPB4.1, a major structural element of the erythrocyte membrane skeleton.
• Protein 4.1 regulates membrane mechanical stability and deformability by stabilizing the spectrin-actin interaction, crucial for maintaining erythrocyte shape.
• It recruits DLG1 to membranes, linking membrane proteins to the cytoskeleton and influencing cell signaling.
• During anaphase, Protein 4.1 is required for the recruitment of the dynein-dynactin complex and NUMA1 to the mitotic cell cortex, facilitating correct spindle orientation and cell division (PubMed:23870127). Related references: PMID:23870127
• Localizes to the cytoplasm, cytoskeleton, cell cortex, and nucleus, reflecting its diverse roles in membrane-associated and nuclear events.
• Post-translational modifications include phosphorylation and the protein contains calmodulin-binding and actin-binding domains, classifying it as a phosphoprotein and glycoprotein. Defects in EPB41 are linked to elliptocytosis and hereditary hemolytic anemia.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 711-810 of human EPB41 (NP_001159477.1), ensuring high specificity for the target. For Western blot, an expected band around 97 kDa should be observed; consider using positive control lysates from human or mouse erythroid cells.
• In IHC-P experiments, validate antibody performance in relevant tissue sections, such as spleen or bone marrow, where erythroid precursors are abundant.
• For ELISA applications, the monoclonal nature of the antibody provides high affinity and low background; optimize dilution and buffer conditions for the specific assay format.
• Since the antibody cross-reacts with both human and mouse, it is suitable for comparative studies across species; however, verify consistency in the relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, serving diagnostic manufacturers, laboratories, and research institutes. Our monoclonal antibodies, including this EPB41 Rabbit mAb, are optimized for applications in membrane cytoskeleton research, cell division studies, and anemia-related diagnostics. Beyond antibodies, CamelBio provides validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your development from concept to clinic.