Anti-EPAS1/HIF2α Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99814

Core Product Specifications and Parameters

Biological Background: EPAS1/HIF2α Function and Localization

• EPAS1, also known as HIF-2α, is a transcription factor that acts as a master regulator of the cellular response to hypoxia.
• It heterodimerizes with ARNT to bind the hypoxia response element (HRE) in target gene promoters, activating genes involved in oxygen homeostasis.
• EPAS1 plays a pivotal role in angiogenesis by regulating vascular endothelial growth factor (VEGF) expression and is essential for vascular development and remodeling.
• It is selectively expressed in endothelial cells and found at highest levels in placenta, lung, and heart tissues.
• The protein localizes predominantly to the nucleus and nuclear speckles, where its transcriptional activity is modulated by post-translational modifications such as hydroxylation and phosphorylation.
• Structurally, EPAS1 belongs to the basic helix-loop-helix (bHLH)-PAS protein family and contains domains critical for DNA binding and dimerization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 678–745) of human EPAS1/HIF2α, which may recognize both full-length and certain truncated forms. Validate detection of the expected 96 kDa band in your experimental system.
• For Western blot (WB), consider using hypoxic cell lysates or endothelial cell lines as positive controls, and optimize primary antibody dilution empirically.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), ensure proper fixation and antigen retrieval; hypoxia-exposed tissues or cells are recommended for signal enrichment.
• Cross-reactivity with mouse and rat is reported, but verify performance in these species under your specific conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. With deep expertise in hypoxia and angiogenesis targets, we support diagnostic manufacturers and researchers from early concept through clinical application. Contact us to source this EPAS1/HIF2α antibody or explore custom solutions for your next project.