Anti-Epac1/RAPGEF3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O95398

Core Product Specifications and Parameters

Biological Background: Epac1/RAPGEF3 Function and Localization

• Encoded by the RAPGEF3 gene, Epac1 (Exchange Protein directly Activated by cAMP 1) is a cAMP-regulated guanine nucleotide exchange factor (GEF) that specifically activates the small GTPases RAP1A and RAP2A.
• As a key mediator of cAMP signaling, Epac1 modulates physiological processes including angiogenesis and the dynamic control of endothelial barrier function through a Rho-independent pathway.
• Epac1 facilitates the assembly of a signaling complex involving PDE3B and PIK3R6, which leads to activation of the PI3K gamma complex, further underscoring its role in compartmentalized cAMP signaling.
• The protein is essential for actin cytoskeleton remodeling at cell-cell junctions, including the formation of stress fibers and junctional actin, thereby maintaining endothelial integrity.
• Epac1 is localized to the endomembrane system and is widely expressed across tissues, with the highest levels observed in adult kidney, heart, thyroid, brain, and fetal kidney.
• Its activity is tightly regulated by direct cAMP binding to its cyclic nucleotide-binding domain, classifying it as a cAMP-binding nucleotide exchange factor.
• Post-translational phosphorylation has been reported, and alternative splicing generates multiple isoforms, expanding its functional diversity.
• Keywords from UniProt include: 3D-structure, Angiogenesis, cAMP, cAMP-binding, Guanine-nucleotide releasing factor, Membrane, Nucleotide-binding, Phosphoprotein, Proteomics identification, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 824-923) of human Epac1; confirm that this region is present in your target isoform if alternative splicing is considered.
• Given the robust tissue-specific expression, consider using lysates from human kidney, heart, or brain as positive controls for Western blotting.
• For immunohistochemistry, validate the antibody on formalin-fixed paraffin-embedded (FFPE) sections, employing antigen retrieval if necessary; include mouse and rat tissues as cross-reactivity controls.
• Perform serial dilutions to determine optimal concentrations for each application; typical starting ranges for rabbit monoclonal antibodies are 1:500–1:2000 for WB and 1:100–1:500 for IHC.
• When running Western blots, the predicted molecular weight is ~104 kDa; use appropriate gel percentages (e.g., 8% SDS-PAGE) and extended transfer times for high-molecular-weight proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop platform for IVD raw-material sourcing, connecting diagnostic developers, research labs, and manufacturers with high-quality antibodies like this Epac1/RAPGEF3 monoclonal. For research targeting cAMP signaling and vascular biology, we offer access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Count on our supply capability to meet your rare-target procurement needs, enabling your projects from concept to clinic.