Anti-EP400 Polyclonal Antibody for WB, ELISA - Q96L91

Core Product Specifications and Parameters

Biological Background: EP400 Function and Localization

• EP400 (E1A-binding protein p400), also known as Domino homolog, CAG repeat protein 32, or Trinucleotide repeat-containing gene 12 protein, is a core component of the NuA4 histone acetyltransferase complex.
• As part of NuA4, EP400 catalyzes acetylation of nucleosomal histones H4 and H2A, altering DNA interactions to facilitate transcriptional activation.
• EP400 participates in the activation of E2F1 and MYC target genes, playing a role in cell proliferation control.
• The ATPase and helicase activities of the NuA4 complex are partly dependent on the interaction of RUVBL1 and RUVBL2 with EP400.
• EP400 is also a subunit of a SWR1-like chromatin remodeling complex that removes histone variant H2A.Z from nucleosomes.
• Localization is primarily nuclear, consistent with its chromatin regulatory function.
• EP400 is ubiquitously expressed across human tissues.
• UniProt annotations highlight ATP-binding, helicase, phosphoprotein, and acetylation modifications.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 90–330 of human EP400; the antibody may preferentially recognize epitopes within this N-terminal region.
• For Western blot, consider using nuclear extracts and high-molecular-weight markers due to EP400’s large size (~343 kDa).
• In ELISA applications, perform antibody titration and optimize coating antigen concentration for best signal-to-noise ratio.
• Validate specificity in the intended cell line or tissue type, as EP400 is ubiquitously expressed but may vary in abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw material partner, offering validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. With this EP400 polyclonal antibody, we support researchers studying chromatin remodeling, transcriptional regulation, and epigenetics. Contact us for customized sourcing of rare targets and technical consultation.