Anti-EP300 Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q09472

Core Product Specifications and Parameters

Biological Background: EP300 Function and Localization

• EP300 encodes the histone acetyltransferase p300, a multidomain transcriptional coactivator belonging to the p300/CBP family. The protein contains a central bromodomain and a histone acetyltransferase (HAT) domain.
• p300 functions as a histone acetyltransferase capable of acetylating all four core histones in nucleosomes, thereby epigenetically tagging chromatin for transcriptional activation (PubMed:23415232, PubMed:23934153, PubMed:8945521). Related references: PMID:23415232 PMID:23934153 PMID:8945521
• It specifically acetylates histone H3 at Lys-18, Lys-27, and Lys-122, and histone H4 at Lys-5 and Lys-12. These modifications promote nucleosome instability and enhance transcriptional initiation (PubMed:21131905, PubMed:23911289, PubMed:23415232, PubMed:37731000). Related references: PMID:21131905 PMID:23911289 PMID:23415232 PMID:37731000
• Beyond histones, p300 acetylates numerous non-histone substrates, including transcription factors (e.g., STAT3, FOXO1), metabolic enzymes (PKM2, PCK1), and DNA repair proteins, modulating their activity, stability, and localization (PubMed:15653507, PubMed:15890677, PubMed:24120661, PubMed:24939902). Related references: PMID:15653507 PMID:15890677 PMID:24120661 PMID:24939902
• The protein localizes to the nucleus, cytoplasm, and chromosomes, reflecting its diverse roles in transcription, signaling, and chromatin structure maintenance.
• p300 participates in circadian rhythm regulation by associating with CLOCK or NPAS2, leading to rhythmic histone acetylation at promoters of clock-controlled genes (PubMed:14645221). Related references: PMID:14645221
• EP300 is associated with multiple diseases: chromosomal rearrangements and mutations are linked to intellectual disability (Rubinstein-Taybi syndrome type 2) and various cancers. The protein also interacts with viral proteins, such as adenovirus E1A and HIV-1 Tat.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 270 amino acids of human EP300, which includes the TAZ1 domain involved in protein-protein interactions. This region is unique to EP300 and may reduce cross-reactivity with the closely related CREBBP protein.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and antibody concentration, given EP300's nuclear/cytoplasmic localization and high molecular weight (~264 kDa).
• ELISA can serve as a quantitative detection platform; validate the assay with recombinant EP300 protein or cell lysates expressing EP300 to ensure specificity.
• Because EP300 is a large protein with multiple domains, denaturing Western blot (WB) conditions may require extended transfer times and careful blocking to detect the full-length band.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs and purified polyclonal antibodies like this anti-EP300 pAb, to support diagnostic manufacturers and research laboratories in developing epigenetic and transcriptional regulation assays. Beyond antibodies, we provide one-stop sourcing for bulk ancillary reagents and access to rare target raw materials, streamlining your path from concept to clinical application. Our technical consulting and supply chain management ensure reliable, high-quality material access for every stage of IVD development.