Anti-EOMES Polyclonal Antibody for WB, ELISA - O95936

Core Product Specifications and Parameters

Biological Background: EOMES Function and Localization

• EOMES encodes a T-box transcription factor (TBR-2) essential for embryonic development, including trophoblast differentiation, gastrulation, and mesoderm/endoderm lineage specification.
• It is critical for brain development, controlling proliferation of intermediate progenitor cells in the cerebral cortex.
  Related references:
  PMID:17353897
• Also required for differentiation and migration of unipolar dendritic brush cells in the cerebellum.
  Related references:
  PMID:33488348
• In the immune system, EOMES drives the differentiation of CD8+ T-cells and regulates expression of lytic effector molecules, linking it to adaptive immunity.
  Related references:
  PMID:17566017
• The protein localizes to the nucleus, consistent with its DNA-binding transcription factor activity.
• Post-translational modifications include phosphorylation, and alternative splicing yields multiple isoforms; chromosomal rearrangements involving EOMES have been reported.
• Expressed in CD8+ T-cells, with broader developmental expression inferred from its functional roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 463-686) of human EOMES; this region is relatively conserved, but sequence alignment should be checked for cross-reactivity in mouse and rat.
• For Western blotting, a band around 73 kDa is expected; however, due to alternative splicing, additional bands may appear.
• For ELISA, consider using this antibody as a capture or detection reagent after confirming performance with the target antigen.
• When working with mouse or rat samples, validate antibody specificity as cross-reactivity is predicted but not guaranteed for all applications.
• Standard immunodetection precautions apply: use appropriate blocking buffers and controls to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs streamlined access to validated IVD raw materials, including polyclonal antibodies like this anti-EOMES reagent for transcriptional regulation studies. Our portfolio supports targets across developmental biology, immunology, and neuroscience, with offerings ranging from optimized antibody pairs and bulk ancillary reagents to custom rare-target sourcing. Contact us to discuss how our one-stop supply model can accelerate your kit development from concept to clinic.