Anti-Envoplakin (EVPL) Rabbit Polyclonal Antibody for WB, ELISA - Q92817

Core Product Specifications and Parameters

Biological Background: Envoplakin Function and Localization

• Envoplakin (EVPL) is a 210 kDa protein, also known as the 210 kDa cornified envelope precursor protein or paraneoplastic pemphigus antigen (p210), and belongs to the plakin family of cytolinkers.
• It serves as a key structural component of the cornified envelope, a specialized structure that forms beneath the plasma membrane of terminally differentiated keratinocytes, providing a protective barrier.
• EVPL may function to anchor the cornified envelope to desmosomes and intermediate filaments, thereby contributing to the mechanical integrity of stratified epithelia.
• Expression of EVPL is strictly limited to stratified squamous epithelia, consistent with its role in epidermal differentiation and barrier formation.
• Subcellularly, EVPL localizes to cell junctions, desmosomes, and the cornified envelope, and is also associated with the cytoskeleton in the cytoplasm.
• Structurally, EVPL contains coiled-coil regions and an SH3 domain, motifs commonly involved in protein-protein interactions and scaffolding.
• Post-translational modifications include phosphorylation, and EVPL is identified as a phosphoprotein, which may regulate its function during keratinocyte differentiation.
• The protein is part of the proteomics identification set and a reference proteome, highlighting its fundamental role in epithelial biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 1784-2033) of human EVPL; this region may be conserved across species, supporting predicted cross-reactivity with mouse and rat.
• For Western blotting, consider using standard protocols; the predicted molecular weight of EVPL is approximately 232 kDa (or 231.6 kDa according to UniProt), so ensure adequate gel porosity and transfer conditions for high-molecular-weight proteins.
• In ELISA applications, the polyclonal nature may provide robust detection, but appropriate blocking and optimization of coating antigen are recommended.
• This antibody has not been tested in other applications; validate experimentally in the relevant sample system before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-Envoplakin antibody supports researchers and diagnostic developers in skin biology, autoimmune blistering diseases, and epithelial barrier studies. Connect with us to streamline procurement of rare-target reagents and accelerate your project from concept to clinic.