Anti-ENTPD6 Polyclonal Antibody for WB, IHC-P, ELISA - O75354

Core Product Specifications and Parameters

Biological Background: ENTPD6 Function and Localization

• ENTPD6, also known as CD39 antigen-like 2, catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner. It has a strong preference for nucleoside diphosphates, preferentially hydrolyzing GDP, IDP, and UDP, with slower activity towards ATP. Related references: PMID:10948193, PMID:11041856, PMID:14529283
• The membrane-bound form of ENTPD6 localizes to the Golgi apparatus membrane, where it is thought to support glycosylation reactions, while the released or cell membrane form can hydrolyze extracellular nucleotides. Related references: PMID:10948193, PMID:11041856, PMID:14529283
• ENTPD6 is expressed in most tissues, with the highest expression observed in the heart.
• The protein undergoes alternative splicing, and post-translational modifications include glycosylation and disulfide bond formation.
• It contains a transmembrane helix and functions as a signal-anchor, facilitating its integration into membranes.
• ENTPD6 is a member of the ecto-nucleoside triphosphate diphosphohydrolase family, which plays a role in regulating nucleotide-mediated signaling and metabolism.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 61-300 of human ENTPD6 (isoform NP_001304870.1). Therefore, the antibody may recognize multiple splice variants if they share this region; validate specificity in your assay system.
• For Western blot, a band around 53 kDa is expected; use appropriate molecular weight markers and consider loading whole-cell lysates or membrane fractions from target tissues.
• In IHC-P, antigen retrieval conditions may need optimization; heart tissue may serve as a positive control due to high expression.
• ELISA performance can be tested using recombinant ENTPD6 protein or cell lysates; note the antibody's reactivity across human, mouse, and rat.
• Since the antibody is polyclonal, batch-to-batch consistency should be verified for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B platform providing end-to-end IVD raw material solutions, from research-grade antibodies to bulk ancillary reagents. For ENTPD6 research—a target involved in nucleotide metabolism and Golgi function—CamelBio can supply validated antibody pairs, optimized polyclonal antibodies like this one, and related detection reagents to support diagnostic assay development. Additionally, we offer customized sourcing for rare targets and technical consulting to bridge your concept to clinic.