Anti-ENTPD5 Rabbit Polyclonal Antibody for IF/ICC and ELISA - O75356

Core Product Specifications and Parameters

Biological Background: ENTPD5 Function and Localization

• ENTPD5, also known as CD39L4, NTPDase-5, ER-UDPase, GDPase, and UDPase, is a nucleoside diphosphate phosphatase that preferentially hydrolyzes GDP, IDP, and UDP over ADP and CDP. Related references: PMID:10400613, PMID:15698960
• In the endoplasmic reticulum lumen, ENTPD5 clears UDP, an end-product inhibitor of UDP-Glc:glycoprotein glucosyltransferases, thereby promoting protein reglucosylation, glycoprotein folding, and ER quality control.
• ENTPD5 is a member of the ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) family, characterized by conserved apyrase domains and requiring divalent cations such as calcium and magnesium for activity.
• The protein localizes predominantly to the endoplasmic reticulum and is also secreted, consistent with its role in UDP metabolism and glycoprotein processing.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, which are critical for its stability and enzymatic function.
• ENTPD5 expression is highest in adult liver, kidney, prostate, testis, and colon, with weaker expression in other tissues, indicating tissue-specific roles in nucleoside diphosphate homeostasis.
• Initially identified as the proto-oncogene PCPH, ENTPD5 has been implicated in cancer progression, though its primary function is in nucleotide metabolism.

Experimental Guidance and Technical Tips

• The immunogen encompasses a large portion of ENTPD5 (amino acids 149–428), including the catalytic domain, suggesting the antibody may recognize both native and denatured forms, suitable for ELISA and immunofluorescence.
• For IF/ICC, optimize fixation and permeabilization conditions; as an ER-resident protein, mild detergent treatment is recommended to preserve ER structure.
• Validate antibody specificity in your experimental system using appropriate controls, such as ENTPD5 siRNA knockdown or overexpression cell lines.
• Although not listed, the polyclonal nature may allow compatibility with other applications like Western blotting; pilot testing under your conditions is advised.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing platform for IVD raw materials, CamelBio streamlines the procurement of research and diagnostic reagents, including validated antibodies for targets like ENTPD5 involved in glycoprotein folding and nucleotide metabolism. Whether you need optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or assistance sourcing rare targets, our supply chain and technical team support your entire development workflow from concept to clinic.