Anti-ENTPD5 Rabbit Monoclonal Antibody for WB, ELISA - O75356

Core Product Specifications and Parameters

Biological Background: ENTPD5 Function and Localization

• ENTPD5, also known as CD39L4, NTPDase-5, or PCPH, is an endoplasmic reticulum-resident nucleoside diphosphatase belonging to the ectonucleoside triphosphate diphosphohydrolase family.
• The enzyme preferentially hydrolyzes nucleoside diphosphates such as GDP, IDP, and UDP, with lower activity toward ADP and CDP. Related references: PMID:10400613 PMID:15698960
• In the ER lumen, ENTPD5 clears UDP, a feedback inhibitor of UDP-Glc:glycoprotein glucosyltransferases, thus positively regulating protein reglucosylation, a process critical for glycoprotein folding and quality control.
• Subcellular localization is predominantly the endoplasmic reticulum; however, secretion has also been reported.
• Tissue expression is highest in adult liver, kidney, prostate, testis, and colon, with much weaker expression in other tissues.
• Key features include calcium/magnesium-dependent hydrolase activity, proto-oncogene classification (PCPH), disulfide bond formation, and glycosylation, highlighting roles in cellular signaling and cancer biology.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein spanning amino acids 300–400 of human ENTPD5 (UniProt O75356). For Western blot, expect a band near 48 kDa; loading controls and species-appropriate secondary antibodies should be included.
• Validated applications are WB and ELISA; for ELISA, consider using recombinant ENTPD5 protein as a standard to confirm reactivity.
• Cross-reactivity with mouse and rat enables multi-species studies; however, validate detection sensitivity in each species' relevant tissue lysate prior to quantitative assays.
• The antibody’s performance in other applications (e.g., IHC, IF) has not been characterized; if required, pilot experiments with appropriate antigen retrieval and controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering validated monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and rare target sourcing to support research from concept to clinic. This ENTPD5 rabbit monoclonal antibody, targeting an ER-resident nucleoside diphosphatase involved in glycoprotein folding and quality control, serves as a valuable tool for investigations in ER stress, cancer biology, and metabolic regulation. Contact us to discuss custom sourcing, technical consultations, or to integrate this antibody into your diagnostic or research workflows.