Anti-ENTPD2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5L3

Core Product Specifications and Parameters

Biological Background: ENTPD2 Function and Localization

• ENTPD2, also designated CD39L1 and NTPDase-2, is a member of the ectonucleoside triphosphate diphosphohydrolase family that hydrolyzes extracellular nucleotides.
• Catalyzes the sequential removal of phosphate groups from nucleoside triphosphates (NTPs) and diphosphates (NDPs), with a marked preference for triphosphonucleosides over diphosphonucleosides, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates. Related references: PMID:10510450, PMID:12888562, PMID:16101300
• ATP hydrolysis by ENTPD2 is characterized by rapid ADP accumulation and delayed AMP formation, reflecting limited ADP hydrolase activity; the enzyme does not hydrolyze AMP. Related references: PMID:10510450, PMID:12888562, PMID:16101300
• Alternative splicing produces multiple isoforms, including catalytically inactive variants, which may contribute to functional diversity.
• Localized predominantly on the cell membrane and the endoplasmic reticulum membrane, positioning it for rapid regulation of extracellular nucleotide levels.
• Expressed in brain, placenta, skeletal muscle, kidney, pancreas, heart, ovary, testis, colon, small intestine, and prostate; not detected in adult thymus, spleen, lung, liver, and peripheral blood leukocytes. Related references: PMID:9271669
• The protein structure includes disulfide bonds, N-glycosylation sites, and predicted binding sites for ATP, GTP, calcium, and magnesium, indicating requirements for post-translational modification and divalent cation cofactors for full activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 28–268 of human ENTPD2, a region predicted to be extracellular; this design may favor detection of native, surface-exposed epitopes.
• For Western blot, an expected band around 54 kDa may be observed; post-translational modifications like glycosylation could cause slight mobility shifts, so consider using appropriate controls.
• In immunofluorescence/immunocytochemistry, optimize permeabilization and fixation conditions to effectively label membrane-bound ENTPD2.
• For ELISA applications, confirm antibody reactivity against recombinant or native ENTPD2 under the specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering diagnostic manufacturers and researchers streamlined access to validated antibodies and ancillary reagents. This anti-ENTPD2 polyclonal antibody exemplifies our commitment to supporting nucleotide metabolism and immunology studies with high-quality reagents. Explore our portfolio for monoclonal/polyclonal options, antibody pairs, and custom sourcing solutions to accelerate your assay development.