Anti-ENTPD1/CD39 Polyclonal Antibody for WB, IF, IHC-P - P49961

Core Product Specifications and Parameters

Biological Background: ENTPD1/CD39 Function and Localization

• ENTPD1, also known as CD39, is an ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase1) belonging to the ENTPD family.
• It catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) in a sequential two-step reaction, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates. Related references: PMID:8529670, PMID:8626624, PMID:8955160
• This activity regulates extracellular nucleotide levels; by hydrolyzing proinflammatory ATP and platelet-activating ADP, it blocks platelet aggregation and supports blood flow. Related references: PMID:8955160, PMID:8996251
• The protein localizes to the plasma membrane and caveolae, consistent with its function as an ecto-enzyme.
• Tissue expression includes activated lymphoid cells, endothelial tissues, and high levels in placenta, lung, skeletal muscle, and kidney. Related references: PMID:7930580, PMID:8955160
• Post-translational modifications include glycosylation, disulfide bonds, and palmitoylation, which are important for membrane anchoring and enzymatic function.
• Mutations in ENTPD1 are associated with hereditary spastic paraplegia (SPG64).
• Alternative splicing produces multiple isoforms of the protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-180) which includes part of the extracellular domain; this design may favor detection of surface-localized or full-length ENTPD1/CD39.
• For western blotting, the predicted molecular weight of ~58 kDa (unmodified) should be validated in the relevant sample system, as glycosylation may cause a higher apparent molecular weight.
• When using immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC, IF-P), consider antigen retrieval methods and validate with appropriate controls, given the membrane-bound localization of the target.
• The reported cross-reactivity with mouse and rat makes this antibody potentially suitable for comparative studies; however, verify species-specific reactivity in your experimental setup.
• For ELISA applications, optimize coating antigen and detection conditions for best performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-ENTPD1/CD39 product supports research into purinergic signaling, immunology, and vascular biology. Leverage our supply capabilities to streamline your diagnostic development from concept to clinic.